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Unger, K. ; Malisch, E. ; Thomas, G.* ; Braselmann, H. ; Walch, A.K. ; Jackl, G. ; Lewis, P.* ; Lengfelder, E.* ; Bogdanova, T.* ; Wienberg, J.* ; Zitzelsberger, H.

Array CGH demonstrates characteristic aberration signatures in human papillary thyroid carcinomas governed by RET/PTC.

Oncogene 27, 4592-4602 (2008)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
The aim of this study is to investigate additional genetic alterations in papillary thyroid carcinomas (PTCs) with known RET/PTC rearrangements. We applied array-based comparative genomic hybridization (array CGH) to 33 PTC (20 PTC from adults, 13 post-Chernobyl PTC from children) with known RET/PTC status. Principal component analysis and hierarchical cluster analysis identified cases with similar aberration patterns. Significant deviations between tumour-groups were obtained by statistical testing (Fisher's exact test in combination with Benjamini-Hochberg FDR-controlling procedure). FISH analysis on FFPE sections was applied to validate the array CGH data. Deletions were found more frequently in RET/PTC-positive and RET/PTC-negative tumours than amplifications. Specific aberration signatures were identified that discriminated between RET/PTC-positive and RET/PTC-negative cases (aberrations on chromosomes 1p, 3q, 4p, 7p, 9p/q, 10q, 12q, 13q and 21q). In addition, childhood and adult RET/PTC-positive cases differ significantly for a deletion on the distal part of chromosome 1p. There are additional alterations in RET/PTC-positive tumours, which may act as modifiers of RET activation. In contrast, alterations in RET/PTC-negative tumours indicate alternative routes of tumour development. The data presented serve as a starting point for further studies on gene expression and function of genes identified in this study.
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Publication type Article: Journal article
Document type Scientific Article
Keywords papillary thyroid carcinoma; array CGH; RET/PTC; candidate genes; tumour development; aberration signature
ISSN (print) / ISBN 0950-9232
e-ISSN 0950-9232
Journal Oncogene
Quellenangaben Volume: 27, Issue: 33, Pages: 4592-4602 Article Number: , Supplement: ,
Publisher Springer
Reviewing status Peer reviewed
Institute(s) Institute of Molecular Radiation Biology (IMS)
Institute of Pathology (PATH)
Institute of Radiation Biology (ISB)
Research Unit Analytical Pathology (AAP)