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Open Access Green as soon as Postprint is submitted to ZB.
High-throughput DNA methylation analysis in anorexia nervosa confirms TNXB hypermethylation.
World J. Biol. Psychiatry 19, 187-199 (2018)
Objectives: Patients with anorexia nervosa (AN) are ideally suited to identify differentially methylated genes in response to starvation.Methods: We examined high-throughput DNA methylation derived from whole blood of 47 females with AN, 47 lean females without AN and 100 population-based females to compare AN with both controls. To account for different cell type compositions, we applied two reference-free methods (FastLMM-EWASher, RefFreeEWAS) and searched for consensus CpG sites identified by both methods. We used a validation sample of five monozygotic AN-discordant twin pairs.Results: Fifty-one consensus sites were identified in AN vs. lean and 81 in AN vs. population-based comparisons. These sites have not been reported in AN methylation analyses, but for the latter comparison 54/81 sites showed directionally consistent differential methylation effects in the AN-discordant twins. For a single nucleotide polymorphism rs923768 in CSGALNACT1 a nearby site was nominally associated with AN. At the gene level, we confirmed hypermethylated sites at TNXB. We found support for a locus at NR1H3 in the AN vs. lean control comparison, but the methylation direction was opposite to the one previously reported.Conclusions: We confirm genes like TNXB previously described to comprise differentially methylated sites, and highlight further sites that might be specifically involved in AN starvation processes.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Anorexia Nervosa ; Dna Methylation ; Eating Disorder ; Epigenome-wide Association Study ; Starvation; Genome-wide Association; Chondroitin Sulfate N-acetylgalactosaminyltransferase-1; Liver X Receptor; Tenascin-x; Eating-disorders; Gene; Epigenetics; Polymorphism; Metabolism; Cartilage
ISSN (print) / ISBN
1562-2975
e-ISSN
1814-1412
Quellenangaben
Volume: 19,
Issue: 3,
Pages: 187-199
Publisher
Taylor & Francis
Publishing Place
Abingdon
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Epidemiology II (EPI2)