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Uniqueness in multispectral constant-wave epi-illumination imaging.
Opt. Lett. 41, 3098-3101 (2016)
Multispectral tissue imaging based on optical cameras and continuous-wave tissue illumination is commonly used in medicine and biology. Surprisingly, there is a characteristic absence of a critical look at the quantities that can be uniquely characterized from optically diffuse matter by multispectral imaging. Here, we investigate the fundamental question of uniqueness in epi-illumination measurements from turbid media obtained at multiple wavelengths. By utilizing an analytical model, tissue-mimicking phantoms, and an in vivo imaging experiment we show that independent of the bands employed, spectral measurements cannot uniquely retrieve absorption and scattering coefficients. We also establish that it is, nevertheless, possible to uniquely quantify oxygen saturation and the Mie scattering power-a previously undocumented uniqueness condition.
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Publication type
Article: Journal article
Document type
Scientific Article
Language
english
Publication Year
2016
HGF-reported in Year
2016
ISSN (print) / ISBN
0146-9592
e-ISSN
1539-4794
Journal
Optics Letters
Quellenangaben
Volume: 41,
Issue: 13,
Pages: 3098-3101
Publisher
Optical Society of America (OSA)
Reviewing status
Peer reviewed
Institute(s)
Institute of Biological and Medical Imaging (IBMI)
POF-Topic(s)
30205 - Bioengineering and Digital Health
Research field(s)
Enabling and Novel Technologies
PSP Element(s)
G-505500-001
WOS ID
WOS:000379667800052
PubMed ID
27367111
Erfassungsdatum
2016-07-09