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Open Access Green as soon as Postprint is submitted to ZB.
Studying axonal regeneration by laser microsurgery and high-resolution videomicroscopy.
Methods Mol. Biol. 1451, 271-280 (2016)
Heterogeneous and unpredictable environmental insult, disease, or trauma can affect the integrity and function of neuronal circuits, leading to irreversible neural dysfunction. The peripheral nervous system can robustly regenerate axons after damage to recover the capacity to transmit sensory information to the brain. The mechanisms that allow axonal repair remain incompletely understood. Here we present a preparation in zebrafish that combines laser microsurgery of sensory axons and videomicroscopy of neurons in multicolor transgenic specimens. This simple protocol allows controlled damage of axons and dynamic high-resolution visualization and quantification of repair.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Fluorescent Protein ; Live Imaging ; Microscopy ; Zebrafish
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 1451,
Pages: 271-280
Publisher
Springer
Publishing Place
Berlin [u.a.]
Reviewing status
Peer reviewed