PuSH - Publication Server of Helmholtz Zentrum München: A Raf kinase inhibitor demonstrates antiviral activities both<em> in vitro</em> and <em>in vivo</em> against different genotypes of virulent Newcastle disease virus.

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Yin, R.* ; Liu, X.* ; Zhang, P.* ; Chen, Y.* ; Xie, G.* ; Ai, L.* ; Xue, C.* ; Qian, J.* ; Bi, Y.* ; Chen, J.* ; Sun, Y.* ; Stöger, T. ; Ding, Z.*

A Raf kinase inhibitor demonstrates antiviral activities both in vitro and in vivo against different genotypes of virulent Newcastle disease virus.

Antiviral Res. 133, 140-144 (2016)

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Open Access Green as soon as Postprint is submitted to ZB.

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Newcastle disease (ND) is still one of the major plagues of birds worldwide. Combat actions are limited to vaccines, highlighting the urgent need for new and amply available antiviral drugs. Previous results have shown that Newcastle disease virus (NDV) downregulates the intracellular Raf kinase inhibitor protein (RKIP) expression for efficient replication, suggesting that this molecular may be a suitable target for antiviral intervention. In the present work, we investigated whether or not the Raf kinase inhibitor V (RKIV), which functions in the same way as RKIP by targeting the intracellular Raf kinase, is able to suppress the propagation of enzootic virulent NDV in vitro and in vivo. In vitro antiviral activity of RKIV was assessed by cell-based assay, and in vivo activity was determined in the chicken model. Our results clearly showed that RKIV treatment protected the cells from NDV-induced CPE with the effective concentrations on nM level, and inhibited virus replication in the lungs of infected chickens in a dose-dependent manner and protected chickens from the lethal infection by NDV. Thus, we conclude that the Raf kinase inhibitor compound RKIV, by inhibiting the host cellular target Raf kinase, might be very promising as a new class of antivirals against the enzootic virulent NDV infection.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Antivirals ; Newcastle Disease Virus ; Raf Kinase ; Raf Kinase Inhibitor V; Influenza-virus; Resistant Variants; Broad-spectrum; Propagation; Replication; Activation; Expression; Infection; Vaccine; Protein

ISSN (print) / ISBN 0166-3542

e-ISSN 1872-9096

Journal Antiviral Research

Quellenangaben Volume: 133, Pages: 140-144

Publisher Elsevier

Publishing Place Amsterdam

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Lung Health and Immunity (LHI)