Home
Deutsch
Advanced Search
Browse by ...
Publication overview
Contact persons
Help
Publ. Version/Full Text
Postprint
DOI
PMC
 |
Open Access Green |
as soon as is submitted to ZB.
Environmental and mucosal microbiota and their role in childhood asthma.
Allergy 72, 109-119 (2017)
BACKGROUND: High microbial diversity in the environment has been associated with lower asthma risk, particularly in children exposed to farming. It remains unclear whether this effect operates through an altered microbiome of the mucosal surfaces of the airways. METHODS: DNA from mattress dust and nasal samples of 86 school-age children was analyzed by 454-pyrosequencing of the 16S rRNA gene fragments. Based on operational taxonomic units bacterial diversity and composition was related to farm exposure and asthma status. RESULTS: Farm exposure was positively associated with bacterial diversity in mattress dust samples as determined by richness (p=8.1 * 10(-6) ) and Shannon index (p=1.3 * 10(-5) ). Despite considerable agreement of richness between mattress and nasal samples, the association of richness with farming in nasal samples was restricted to a high gradient of farm exposure, i.e. exposure to cows and straw versus no exposure at all. In mattress dust the genera Clostridium, Facklamia, an unclassified genus within the family of Ruminococcaceae and six OTUs were positively associated with farming. Asthma was inversely associated with richness (aOR= 0.48 [0.22-1.02]) and Shannon Index (aOR=0.41 [0.21-0.83]) in mattress dust and to a lower extent in nasal samples (richness aOR 0.63[0.38-1.06], Shannon Index aOR= 0.66 [0.39-1.12]). CONCLUSION: The stronger inverse association of asthma and bacterial diversity in mattress dust as compared to nasal samples suggests microbial involvement beyond mere colonization of the upper airways. Whether inhalation of metabolites of environmental bacteria contributes to this phenomenon should be the focus of future research.
Altmetric
Additional Metrics?
Edit extra informations
Login
Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Asthma ; Epidemiology ; Microbiome ; Pediatrics; Gut Microbiota; House-dust; Bacterial; Allergy; Milk; Community; Exposure; Children; Skin; Raw
ISSN (print) / ISBN
0105-4538
e-ISSN
1398-9995
Journal
Allergy
Quellenangaben
Volume: 72,
Issue: 1,
Pages: 109-119
Publisher
Wiley
Publishing Place
Hoboken
Non-patent literature
Publications
Reviewing status
Peer reviewed