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CRISPR-Cas9 enables conditional mutagenesis of challenging loci.
Sci. Rep. 6:32326 (2016)
The International Knockout Mouse Consortium (IKMC) has produced a genome-wide collection of 15,000 isogenic targeting vectors for conditional mutagenesis in C57BL/6N mice. Although most of the vectors have been used successfully in murine embryonic stem (ES) cells, there remain a set of nearly two thousand genes that have failed to target even after several attempts. Recent attention has turned to the use of new genome editing technology for the generation of mutant alleles in mice. Here, we demonstrate how Cas9-assisted targeting can be combined with the IKMC targeting vector resource to generate conditional alleles in genes that have previously eluded targeting using conventional methods.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
One-step Generation; Homologous Recombination; Gene-function; Genome; Cas9; Consortium; Resource; System
Language
english
Publication Year
2016
HGF-reported in Year
2016
ISSN (print) / ISBN
2045-2322
e-ISSN
2045-2322
Journal
Scientific Reports
Quellenangaben
Volume: 6,
Article Number: 32326
Publisher
Nature Publishing Group
Publishing Place
London
Reviewing status
Peer reviewed
Institute(s)
Institute of Developmental Genetics (IDG)
Institute of Molecular Toxicology and Pharmacology (TOX)
Institute of Molecular Toxicology and Pharmacology (TOX)
POF-Topic(s)
30204 - Cell Programming and Repair
30203 - Molecular Targets and Therapies
30203 - Molecular Targets and Therapies
Research field(s)
Genetics and Epidemiology
Enabling and Novel Technologies
Enabling and Novel Technologies
PSP Element(s)
G-500500-001
G-505200-001
G-505200-001
PubMed ID
27580957
WOS ID
WOS:000382367900001
Scopus ID
84984868418
Erfassungsdatum
2016-09-05