PuSH - Publication Server of Helmholtz Zentrum München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Giroud, M.* ; Pisani, D.F.* ; Karbiener, M.* ; Barquissau, V.* ; Ghandour, R.A.* ; Tews, D.* ; Fischer-Posovszky, P.* ; Chambard, J.C.* ; Knippschild, U.* ; Niemi, T.* ; Taittonen, M.* ; Nuutila, P.* ; Wabitsch, M.* ; Herzig, S. ; Virtanen, K.A.* ; Langin, D.* ; Scheideler, M. ; Amri, E.Z.*

miR-125b affects mitochondrial biogenesis and impairs brite adipocyte formation and function.

Mol. Metab. 5, 615-25 (2016)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
OBJECTIVE: In rodents and humans, besides brown adipose tissue (BAT), islands of thermogenic adipocytes, termed "brite" (brown-in-white) or beige adipocytes, emerge within white adipose tissue (WAT) after cold exposure or β3-adrenoceptor stimulation, which may protect from obesity and associated diseases. microRNAs are novel modulators of adipose tissue development and function. The purpose of this work was to characterize the role of microRNAs in the control of brite adipocyte formation. METHODS/RESULTS: Using human multipotent adipose derived stem cells, we identified miR-125b-5p as downregulated upon brite adipocyte formation. In humans and rodents, miR-125b-5p expression was lower in BAT than in WAT. In vitro, overexpression and knockdown of miR-125b-5p decreased and increased mitochondrial biogenesis, respectively. In vivo, miR-125b-5p levels were downregulated in subcutaneous WAT and interscapular BAT upon β3-adrenergic receptor stimulation. Injections of an miR-125b-5p mimic and LNA inhibitor directly into WAT inhibited and increased β3-adrenoceptor-mediated induction of UCP1, respectively, and mitochondrial brite adipocyte marker expression and mitochondriogenesis. CONCLUSION: Collectively, our results demonstrate that miR-125b-5p plays an important role in the repression of brite adipocyte function by modulating oxygen consumption and mitochondrial gene expression.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
5.363
1.419
37
39
Tags
Annotations
Special Publikation
Hide on homepage

Edit extra information
Edit own tags
Private
Edit own annotation
Private
Hide on publication lists
on hompage
Mark as special
publikation
Publication type Article: Journal article
Document type Scientific Article
Keywords Brite Adipocyte ; Mitochondriogenesis ; White Adipocyte ; Mir-125b-5p; Brown Adipose-tissue; In-vivo; Adipogenic Differentiation; Fat Differentiation; Uncoupling Protein; Gene-expression; Microarray Data; Adult Mice; Beige Fat; White
Language german
Publication Year 2016
HGF-reported in Year 2016
ISSN (print) / ISBN 2212-8778
e-ISSN 2212-8778
Journal Molecular Metabolism
Quellenangaben Volume: 5, Issue: 8, Pages: 615-25 Article Number: , Supplement: ,
Publisher Elsevier
Publishing Place Amsterdam
Reviewing status Peer reviewed
Institute(s) Institute of Diabetes and Cancer (IDC)
POF-Topic(s) 90000 - German Center for Diabetes Research
Research field(s) Helmholtz Diabetes Center
PSP Element(s) G-501900-251
G-501900-252
PubMed ID 27656399
DOI 10.1016/j.molmet.2016.06.005
WOS ID WOS:000386882400003
Scopus ID 84989949578
Erfassungsdatum 2016-10-07
[➜Report correction]