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Peters, S.* ; Kronseder, A.* ; Karrasch, S. ; Neff, P.A.* ; Haaks, M.* ; Koczulla, A.R.* ; Reinhold, P.* ; Nowak, D.* ; Jörres, R.A.*

Hydrogen peroxide in exhaled air: A source of error, a paradox and its resolution.

ERJ Open Res. 2:00052-2015 (2016)
Publ. Version/Full Text DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The concentration of hydrogen peroxide (H2O2) in exhaled air has been reported to be elevated in asthma and chronic obstructive pulmonary disease (COPD), but results are inconsistent and difficult to reproduce. As H2O2 occurs in ambient air, we examined its association with exhaled H2 O2 in human subjects. Exhaled breath condensate (EBC) of 12 COPD patients and nine healthy control subjects was collected either with an inhalation filter (efficiency 81%) or without. Ambient air condensate (AAC) was collected in parallel and samples were analysed for H2O2. Additionally, ambient H2O2 was recorded by an atmospheric measuring device (online fluorometric measurement). H2 O2 concentration in AAC was significantly higher (p<0.001) than in EBC. AAC variations were concordant with the data from the atmospheric measuring instrument. In both subjects’ groups, the inhalation filter reduced H2O2 values (p<0.01). Despite generally low levels in exhaled air, analysis by a mathematical model revealed a contribution from endogenous H2O2 production. The low H2O2 levels in exhaled air are explained by the reconditioning of H2O2-containing inhaled air in the airways. Inhaled H2O2 may be one factor in the heterogeneity and limited reproducibility of study results. A valid determination of endogenous H2 O2 production requires inhalation filters.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
ISSN (print) / ISBN 2312-0541
e-ISSN 2312-0541
Journal ERJ Open Research
Quellenangaben Volume: 2, Issue: 2, Pages: , Article Number: 00052-2015 Supplement: ,
Publisher European Respiratory Society
Publishing Place [S.l.]
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Epidemiology (EPI)