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Open Access Green as soon as Postprint is submitted to ZB.
Increasing the chemical-shift dispersion of unstructured proteins with a covalent lanthanide shift reagent.
Angew. Chem.-Int. Edit. 55, 14847-14851 (2016)
The study of intrinsically disordered proteins (IDPs) by NMR often suffers from highly overlapped resonances that prevent unambiguous chemical-shift assignments, and data analysis that relies on well-separated resonances. We present a covalent paramagnetic lanthanide-binding tag (LBT) for increasing the chemical-shift dispersion and facilitating the chemical-shift assignment of challenging, repeat-containing IDPs. Linkage of the DOTA-based LBT to a cysteine residue induces pseudo-contact shifts (PCS) for resonances more than 20 residues from the spin-labeling site. This leads to increased chemical-shift dispersion and decreased signal overlap, thereby greatly facilitating chemical-shift assignment. This approach is applicable to IDPs of varying sizes and complexity, and is particularly helpful for repeat-containing IDPs and low-complexity regions. This results in improved efficiency for IDP analysis and binding studies.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Nmr ; Chemical-shift Dispersion ; Intrinsically Disordered Proteins ; Lanthanides ; Pseudo-contact Shifts; Intrinsically Disordered Proteins; Backbone Resonance Assignment; Nmr-spectroscopy; Unfolded Proteins; Biomolecular Nmr; Low-complexity; Regions; Binding; Fus; Transportin
Language
Publication Year
2016
HGF-reported in Year
2016
ISSN (print) / ISBN
1433-7851
e-ISSN
1521-3773
Quellenangaben
Volume: 55,
Issue: 47,
Pages: 14847-14851
Publisher
Wiley
Publishing Place
Weinheim
Reviewing status
Peer reviewed
Institute(s)
Institute of Structural Biology (STB)
POF-Topic(s)
30505 - New Technologies for Biomedical Discoveries
30203 - Molecular Targets and Therapies
30203 - Molecular Targets and Therapies
Research field(s)
Enabling and Novel Technologies
PSP Element(s)
G-552800-001
G-503000-001
G-503000-001
PubMed ID
27763708
WOS ID
WOS:000388252700067
Scopus ID
84995562570
Erfassungsdatum
2016-10-24