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Buschmann, H. ; Fabri, C.O. ; Hauptmann, M. ; Hutzler, P. ; Laux, Th.* ; Lloyd, C.W.* ; Schäffner, A.

Helical growth of the Arabidopsis mutant tortifolia1 reveals a plant-specific microtubule-associated protein.

Curr. Biol. 14, 1515-1521 (2004)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
Plants can grow straight or in the twisted fashion exhibited by the helical growth of some climbing plants. Analysis of helical-growth mutants from Arabidopsis has indicated that microtubules are involved in the expression of the helical phenotype. Arabidopsis mutants growing with a right-handed twist have been reported to have cortical microtubules that wind around the cell in left-handed helices and vice versa [1–3] . Microtubular involvement is further suspected from the finding that some helical mutants are caused by single amino acid substitutions in α-tubulin and because of the sensitivity of the growth pattern to anti-microtubule drugs. Insight into the roles of microtubules in organ elongation is anticipated from analyses of genes defined by helical mutations [4] . We investigated the helical growth of the Arabidopsis mutant tortifolia1/spiral2 (tor1/spr2), which twists in a right-handed manner, and found that this correlates with a complex reorientation of cortical microtubules. TOR1 was identified by a map-based approach; analysis of the TOR1 protein showed that it is a member of a novel family of plant-specific proteins containing N-terminal HEAT repeats. Recombinant TOR1 colocalizes with cortical microtubules in planta and binds directly to microtubules in vitro. This shows that TOR1 is a novel, plant-specific microtubule-associated protein (MAP) that regulates the orientation of cortical microtubules and the direction of organ growth.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
ISSN (print) / ISBN 0960-9822
e-ISSN 1879-0445
Journal Current Biology
Quellenangaben Volume: 14, Issue: 16, Pages: 1515-1521 Article Number: , Supplement: ,
Publisher Elsevier
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Pathology (PATH)
Institute of Biochemical Plant Pathology (BIOP)