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Herold, T. ; Metzeler, K.H. ; Vosberg, S. ; Hartmann, L. ; Jurinovic, V.* ; Opatz, S. ; Konstandin, N.P.* ; Schneider, S.* ; Zellmeier, E.* ; Ksienzyk, B.* ; Graf, A.* ; Krebs, S.* ; Blum, H.* ; Cristina Sauerland, M.* ; Büchner, T.* ; Berdel, W.E.* ; Wörmann, B.J.* ; Mansmann, U.* ; Hiddemann, W. ; Bohlander, S.K.* ; Spiekermann, K. ; Greif, P.A.

Acute myeloid leukemia with del(9q) is characterized by frequent mutations of NPM1, DNMT3A, WT1 and low expression of TLE4.

Genes Chromosomes Cancer 56, 75-86 (2017)
Postprint DOI PMC
Open Access Green
Deletions of the long arm of chromosome 9 [del(9q)] are a rare but recurring aberration in acute myeloid leukemia (AML). Del(9q) can be found as the sole abnormality or in combination with other cytogenetic aberrations such as t(8;21) and t(15;17). TLE1 and TLE4 were identified to be critical genes contained in the 9q region. We performed whole exome sequencing of 5 patients with del(9q) as the sole abnormality followed by targeted amplicon sequencing of 137 genes of 26 patients with del(9q) as sole or combined with other aberrations. We detected frequent mutations in NPM1 (10/26; 38%), DNMT3A (8/26; 31%), and WT1 (8/26; 31%) but only few FLT3-ITDs (2/26; 8%). All mutations affecting NPM1 and DNMT3A were exclusively identified in patients with del(9q) as the sole abnormality and were significantly more frequent compared to 111 patients classified as intermediate-II according to the European LeukemiaNet (10/14, 71% vs. 22/111, 20%; P < 0.001, 8/14, 57% vs. 26/111, 23%; P = 0.02). Furthermore, we identified DNMT3B to be rarely but recurrently targeted by truncating mutations in AML. Gene expression analysis of 13 patients with del(9q) and 454 patients with normal karyotype or various cytogenetic aberrations showed significant down regulation of TLE4 in patients with del(9q) (P = 0.02). Interestingly, downregulation of TLE4 was not limited to AML with del(9q), potentially representing a common mechanism in AML pathogenesis. Our comprehensive genetic analysis of the del(9q) subgroup reveals a unique mutational profile with the frequency of DNMT3A mutations in the del(9q) only subset being the highest reported so far in AML, indicating oncogenic cooperativity.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords European Leukemianet; Prognostic Impact; Dna Methylation; Cebpa Mutations; Aml; Flt3; Identification; Genes; Recommendations; Lymphomagenesis
ISSN (print) / ISBN 1045-2257
e-ISSN 1098-2264
Journal Genes, Chromosomes and Cancer
Quellenangaben Volume: 56, Issue: 1, Pages: 75-86 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place Hoboken
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) CCG Pathogenesis of Acute Myeloid Leukemia (KKG-KPL)