Home
Deutsch
Advanced Search
Browse by ...
Publication overview
Contact persons
Help
Publ. Version/Full Text
DOI
PMC
 |
Free by publisher |
as soon as is submitted to ZB.
Exhaust air dust monitoring is superior to soiled bedding sentinels for the detection of pasteurella pneumotropica in individually ventilated cage systems.
J. Amer. Assoc. Lab. Anim. Sci. 55, 775-781 (2016)
Reliable detection of unwanted organisms is essential for meaningful health monitoring in experimental animal facilities. Currently, most rodents are housed in IVC systems, which prevent the aerogenic transmission of pathogens between cages. Typically soiled-bedding sentinels (SBS) exposed to soiled bedding collected from a population of animals within an IVC rack are tested as representatives, but infectious agents often go undetected due to inefficient transmission. Pasteurellaceae are among the most prevalent bacterial pathogens isolated from experimental mice, and the failure of SBS to detect these bacteria is well established. In this study, we investigated whether analysis of exhaust air dust (EAD) samples by using a sensitive and specific real-time PCR assay is superior to conventional SBS monitoring for the detection of Pasteurella pneumotropica (Pp) infections. In a rack with a known prevalence of Pp-positive mice, weekly EAD sampling was compared with the classic SBS method over 3 mo. In 6 rounds of testing, with a prevalence of 5 infected mice in each of 7 cages in a rack of 63 cages, EAD PCR detected Pp at every weekly time point; SBS failed to detect Pp in all cases. The minimal prevalence of Pp-infected mice required to obtain a reliable positive result by EAD PCR testing was determined to be 1 in 63 cages. Reliable detection of Pp was achieved after only 1 wk of exposure. Analysis of EAD samples by real-time PCR assay provides a sensitive, simple, and reliable approach for Pp identification in laboratory mice.
Altmetric
Additional Metrics?
Edit extra informations
Login
Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Polymerase-chain-reaction; Laboratory Mice; Natural Pathogens; Fur Mites; Pcr Assay; Identification; Rats; Infection; Rodents; Efficacy
ISSN (print) / ISBN
1060-0558
e-ISSN
1559-6109
Quellenangaben
Volume: 55,
Issue: 6,
Pages: 775-781
Publisher
American Association for Laboratory Animal Science (AALAS)
Publishing Place
Memphis
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
CF Comparative Medicine (CF-Comparative Medicine)