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Azimzadeh, O. ; Scherthan, H.* ; Sarioglu, H. ; Barjaktarovic, Z. ; Conrad, M. ; Vogt, A.* ; Calzada-Wack, J. ; Neff, F. ; Aubele, M. ; Buske, C. ; Atkinson, M.J. ; Tapio, S.

Rapid proteomic remodeling of cardiac tissue caused by total body ionizing radiation.

Proteomics 11, 3299-3311 (2011)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Accidental nuclear scenarios lead to environmental contamination of unknown level. Immediate radiation-induced biological responses that trigger processes leading to adverse health effects decades later are not well understood. A comprehensive proteomic analysis provides a promising means to identify and quantify the initial damage after radiation exposure. Early changes in the cardiac tissue of C57BL/6 mice exposed to total body irradiation were studied, using a dose relevant to both intentional and accidental exposure (3 Gy gamma ray). Heart tissue protein lysates were analyzed 5 and 24 h after the exposure using isotope-coded protein labeling (ICPL) and 2-dimensional difference-in-gel-electrophoresis (2-D DIGE) proteomics approaches. The differentially expressed proteins were identified by LC-ESI-MS-MS. Both techniques showed similar functional groups of proteins to be involved in the initial injury. Pathway analyses indicated that total body irradiation immediately induced biological responses such as inflammation, antioxidative defense, and reorganization of structural proteins. Mitochondrial proteins represented the protein class most sensitive to ionizing radiation. The proteins involved in the initial damage processes map to several functional categories involving cardiotoxicity. This prompts us to propose that these early changes are indicative of the processes that lead to an increased risk of cardiovascular disease after radiation exposure.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Cardiac disease; Cell biology; 2-D DIGE; Ionizing irradiation; Isotope-coded protein labeling; Oxidative stress
ISSN (print) / ISBN 1615-9853
e-ISSN 1615-9861
Journal Proteomics
Quellenangaben Volume: 11, Issue: 6, Pages: 3299-3311 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place Weinheim
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Radiation Biology (ISB)
CF Metabolomics & Proteomics (CF-MPC)
Institute of Developmental Genetics (IDG)
Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)
Institute of Pathology (PATH)