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Differential response of normal and transformed mammary epithelial cells to combined treatment of anti-miR-21 and radiation.
Int. J. Radiat. Biol. 93, 361-372 (2017)
Publ. Version/Full Text
Research data
DOI
PMC
Purpose: MicroRNA miR-21 has emerged as a therapeutic target in the treatment of breast cancer. This study was designed to compare the responses of breast cancer cells and non-transformed breast epithelial cells to a combined regimen of miR-21 inhibition and radiation. Materials and methods: The MDA-MB-361 (breast cancer) and MCF-10A (non-transformed mammary epithelial) cell lines were used for the comparison in this in vitro study. The stable knockdown of miR-21 was performed by using lentiviral approach. The response of the cells was monitored 4, 24 and 48 h after the irradiation with 0.25 and 2.5 Gy, using sham-irradiated cells as controls. The response of the cells was established by performing various functional assays – cell viability and cell attachment, clonogenic survival, cell cycle analysis and 3D microtissue formation. Results: The knockdown of miR-21 induced significant increase in apoptosis and growth delay in MDA-MB-361 cancer cells compared to non-transformed MCF-10A cells. After combined radiation and anti-miR-21 treatment, MDA-MB-361 cells show reduced cell growth and viability what is presented in their inability to form colonies. MCF-10A cells were not as sensitive to the combined treatment and that has also been confirmed with colony forming assay. Conclusions: Cellular response to a combined treatment of anti-miR-21 and radiation is different between cancer and non-cancer cells which highly support the idea of linking miR-21 inhibitor and radiation treatment in the future therapeutic approaches for breast cancer.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Cellular Response ; Mcf-10a ; Mir-21 ; Radiation Oncology; Breast-cancer; Tumor-growth; Therapy; Mir-21; Microrna-21; Expression; Proliferation; Tumorigenesis; Resistance; Addiction
ISSN (print) / ISBN
0955-3002
e-ISSN
1362-3095
Quellenangaben
Volume: 93,
Issue: 4,
Pages: 361-372
Publisher
Informa Healthcare
Publishing Place
Abingdon
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Radiation Biology (ISB)