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Open Access Green as soon as Postprint is submitted to ZB.
Use of chemical mutagenesis in mouse embryonic stem cells.
Methods Mol. Biol. 329, 397-407 (2006)
The chemical mutagenesis of mouse embryonic stem (ES) cells is an approach complementary to chemical mutagenesis of spermatogonia in whole animals. It has great potential to contribute significantly to the generation of a comprehensive collection of multiple alleles for most mammalian genes and to facilitate the progression from gene sequence to gene functional analyses. The general strategy includes the treatment of ES cells with a chemical mutagen and the isolation of individual mutagenized clones that are then cultured in duplicate. Whereas one set of samples is cryopreserved for archiving and subsequent generation of germline chimeras by means of blastocyst injection, the second set is used for genotypeor phenotype-driven screens to identify mutant alleles.
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Publication type
Article: Journal article
Document type
Scientific Article
Editors
Turksen, K.*
Keywords
Chemical mutagenesis; ES cells; genotype screen; N-ethyl-N-nitrosourea (ENU); methanesulfonic acid ethyl ester (EMS); N-nitroso-N-ethylurea; phenotype screen
Language
english
Publication Year
2006
HGF-reported in Year
0
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Conference Title
Embryonic Stem Cell Protocols : Volume 1: Isolation and Characterization
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 329,
Issue: 27,
Pages: 397-407
Series
Embryonic Stem Cell Protocols
Publisher
Springer
Publishing Place
Berlin [u.a.]
Reviewing status
Peer reviewed
Institute(s)
Institute of Experimental Genetics (IEG)
POF-Topic(s)
30201 - Metabolic Health
Research field(s)
Genetics and Epidemiology
PSP Element(s)
G-500600-004
PubMed ID
16846006
Erfassungsdatum
2006-12-31