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Englhard, A.S.* ; Palaras, A.* ; Volgger, V.* ; Stepp, H.* ; Mack, B.* ; Libl, D.* ; Gires, O. ; Betz, C.S.*

Confocal laser endomicroscopy in head and neck malignancies using FITC-labelled EpCAM- and EGF-R-antibodies in cell lines and tumor biopsies.

J. Biophotonics 10, 1365–1376 (2017)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Intraoperative detection of residual malignant cells at tumor margins following excision of primary tumors could help improving surgery and thus patients' outcome. The feasibility of the tumor antigens epidermal growth factor receptor (EGF-R) and epithelial cell adhesion molecule (EpCAM) for antibody-dependent confocal laser scanning endomicroscopy (CLE)-mediated visualization of malignant cells was addressed. Both tumor antigens are highly and frequently expressed in the majority of carcinomas, including head and neck squamous cell carcinomas (HNSCC), and represent prognostic and therapeutic tumor target molecules. FITC-conjugated EGF-R- and EpCAM-specific antibodies served as molecular tools for the detection of antigen-positive cells using the CLE technology. Specificity of both antibodies and their ability to discriminate tumor from non-tumor cells were assessed in vitro with human fibroblasts and PCI-1 HNSCC cell lines, and ex vivo on primary HNSCC samples (n = 11) and healthy mucosa (n = 5). Antigen specificity of the used EpCAM-specific antibody was superior to that of the EGF-R-specific antibody both in vitro and ex vivo (100% vs. 31.25%), and allowed visualization of cellular structures in CLE measurements. These results hold promise for possible future applications in humans.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Cle ; Egf-r ; Endomicroscopy ; Epcam ; Hnscc; Growth-factor-receptor; Embryonic Stem-cells; Cancer In-vivo; Ep-cam; Adhesion Molecule; Breast-cancer; Intraoperative Application; Barretts-esophagus; Colorectal-cancer; Expression
ISSN (print) / ISBN 1864-063X
e-ISSN 1864-0648
Journal Journal of Biophotonics
Quellenangaben Volume: 10, Issue: 10, Pages: 1365–1376 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place Weinheim
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) CCG Personalized Radiotherapy in Head and Neck Cancer (KKG-KRT)