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Kong, H.H.* ; Andersson, B.* ; Clavel, T.* ; Common, J.E.* ; Jackson, S.A.* ; Olson, A.D.* ; Segre, J.A.* ; Traidl-Hoffmann, C.

Performing skin microbiome research: A method to the madness.

J. Invest. Dermatol. 137, 561-568 (2017)
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Growing interest in microbial contributions to human health and disease has increasingly led investigators to examine the microbiome in both healthy skin and cutaneous disorders, including acne, psoriasis, and atopic dermatitis. The need for common language, effective study design, and validated methods is critical for high-quality standardized research. Features, unique to skin, pose particular challenges when conducting microbiome research. This review discusses microbiome research standards and highlights important factors to consider, including clinical study design, skin sampling, sample processing, DNA sequencing, control inclusion, and data analysis.
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Publication type Article: Journal article
Document type Review
Corresponding Author
Keywords OTU, operational taxonomic unit; rRNA, ribosomal RNA; V, variable; 16s Ribosomal-rna; Atopic-dermatitis; Antimicrobial Defense; Cutaneous Bacterial; Minimum Information; Fungal Populations; Molecular Analysis; Sequencing Data; Body Habitats; Diversity
ISSN (print) / ISBN 0022-202X
e-ISSN 1523-1747
Quellenangaben Volume: 137, Issue: 3, Pages: 561-568 Article Number: , Supplement: ,
Publisher Elsevier
Publishing Place New York, NY
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Environmental Medicine (IEM)