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Kremb, S.* ; Müller, C. ; Schmitt-Kopplin, P. ; Voolstra, C.R.*

Bioactive potential of marine macroalgae from the central red sea (Saudi Arabia) assessed by high-throughput imaging-based phenotypic profiling.

Mar. Drugs 15:E80 (2017)

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Marine algae represent an important source of novel natural products. While their bioactive potential has been studied to some extent, limited information is available on marine algae from the Red Sea. This study aimed at the broad discovery of new bioactivities from a collection of twelve macroalgal species from the Central Red Sea. We used imaging-based High-Content Screening (HCS) with a diverse spectrum of cellular markers for detailed cytological profiling of fractionated algal extracts. The cytological profiles for 3 out of 60 algal fractions clustered closely to reference inhibitors and showed strong inhibitory activities on the HIV-1 reverse transcriptase in a single-enzyme biochemical assay, validating the suggested biological target. Subsequent chemical profiling of the active fractions of two brown algal species by ultra-high resolution mass spectrometry (FT-ICR-MS) revealed possible candidate molecules. A database query of these molecules led us to groups of compounds with structural similarities, which are suggested to be responsible for the observed activity. Our work demonstrates the versatility and power of cytological profiling for the bioprospecting of unknown biological resources and highlights Red Sea algae as a source of bioactives that may serve as a starting point for further studies.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Hiv ; High-content Screening ; Algae ; Bioprospecting ; Cytological Profiling ; Natural Products; Cell-cycle Arrest; Natural-product Libraries; Cdk Inhibitors P21; Drug Discovery; Cancer-cells; Brown-algae; Reverse-transcriptase; Organic-matter; Peyssonenyne-b; Ecklonia-cava

ISSN (print) / ISBN 1660-3397

e-ISSN 1660-3397

Journal Marine Drugs

Quellenangaben Volume: 15, Issue: 3, Article Number: E80

Publisher MDPI

Publishing Place Basel

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Research Unit Analytical BioGeoChemistry (BGC)