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In vitro generation of vascular wall-resident multipotent stem cells of mesenchymal nature from murine induced pluripotent stem cells.
Stem Cell Rep. 8, 919-932 (2017)
The vascular wall (VW) serves as a niche for mesenchymal stem cells (MSCs). In general, tissue-specific stem cells differentiate mainly to the tissue type from which they derive, indicating that there is a certain code or priming within the cells as determined by the tissue of origin. Here we report the in vitro generation of VW-typical MSCs from induced pluripotent stem cells (iPSCs), based on a VW-MSC-specific gene code. Using a lentiviral vector expressing the so-called Yamanaka factors, we reprogrammed tail dermal fibroblasts from transgenic mice containing the GFP gene integrated into the Nestin-locus (NEST-iPSCs) to facilitate lineage tracing after subsequent MSC differentiation. A lentiviral vector expressing a small set of recently identified human VW-MSC-specific HOX genes then induced MSC differentiation. This direct programming approach successfully mediated the generation of VW-typical MSCs with classical MSC characteristics, both in vitro and in vivo.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Hox Gene ; Direct Programming ; Induced Pluripotent Stem Cells ; Nestin ; Vascular Wall-derived Mesenchymal Stem Cells; Human Bone-marrow; Stromal Cells; Different Organs; Adipose-tissue; Cord Blood; Hox Codes; Adult; Fetal; Differentiation; Expression
ISSN (print) / ISBN
2213-6711
Journal
Stem Cell Reports
Quellenangaben
Volume: 8,
Issue: 4,
Pages: 919-932
Publisher
Cell Press
Publishing Place
Maryland Heights, MO
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Translational Metabolic Oncology (TMO)