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Fischer, K. ; Ruiz, H.H.* ; Jhun, K.* ; Finan, B. ; Oberlin, D.J.* ; van der Heide, V.* ; Kalinovich, A.V.* ; Petrovic, N.* ; Wolf, Y.* ; Clemmensen, C. ; Shin, A.C.* ; Divanovic, S.* ; Brombacher, F.* ; Glasmacher, E. ; Keipert, S. ; Jastroch, M. ; Nagler, J. ; Schramm, K.-W. ; Medrikova, D. ; Collden, G. ; Woods, S.C.* ; Herzig, S. ; Homann, D.* ; Jung, S.* ; Nedergaard, J.* ; Cannon, B.* ; Tschöp, M.H. ; Müller, T.D. ; Buettner, C.*

Alternatively activated macrophages do not synthesize catecholamines or contribute to adipose tissue adaptive thermogenesis.

Nat. Med. 23, 623-630 (2017)
Postprint Research data DOI PMC
Open Access Green
Adaptive thermogenesis is the process of heat generation in response to cold stimulation. It is under the control of the sympathetic nervous system, whose chief effector is the catecholamine norepinephrine (NE). NE enhances thermogenesis through β3-adrenergic receptors to activate brown adipose tissue and by 'browning' white adipose tissue. Recent studies have reported that alternative activation of macrophages in response to interleukin (IL)-4 stimulation induces the expression of tyrosine hydroxylase (TH), a key enzyme in the catecholamine synthesis pathway, and that this activation provides an alternative source of locally produced catecholamines during the thermogenic process. Here we report that the deletion of Th in hematopoietic cells of adult mice neither alters energy expenditure upon cold exposure nor reduces browning in inguinal adipose tissue. Bone marrow-derived macrophages did not release NE in response to stimulation with IL-4, and conditioned media from IL-4-stimulated macrophages failed to induce expression of thermogenic genes, such as uncoupling protein 1 (Ucp1), in adipocytes cultured with the conditioned media. Furthermore, chronic treatment with IL-4 failed to increase energy expenditure in wild-type, Ucp1(-/-) and interleukin-4 receptor-α double-negative (Il4ra(-/-)) mice. In agreement with these findings, adipose-tissue-resident macrophages did not express TH. Thus, we conclude that alternatively activated macrophages do not synthesize relevant amounts of catecholamines, and hence, are not likely to have a direct role in adipocyte metabolism or adaptive thermogenesis.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Beige Fat; Noradrenaline; Metabolism; Clearance; Mice
Language english
Publication Year 2017
HGF-reported in Year 2017
ISSN (print) / ISBN 1078-8956
e-ISSN 1546-170X
Journal Nature medicine
Quellenangaben Volume: 23, Issue: 5, Pages: 623-630 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Publishing Place New York, NY
Reviewing status Peer reviewed
Institute(s) Institute of Diabetes and Obesity (IDO)
PPM-MEX-Molecular EXposomics (MEX)
Institute of Diabetes and Cancer (IDC)
POF-Topic(s) 30201 - Metabolic Health
90000 - German Center for Diabetes Research
30202 - Environmental Health
Research field(s) Helmholtz Diabetes Center
Environmental Sciences
PSP Element(s) G-502200-001
G-501900-226
G-509100-001
G-501900-251
G-501900-221
DOI 10.1038/nm.4316
WOS ID WOS:000400650700017
Scopus ID 85017520090
PubMed ID 28414329
Erfassungsdatum 2017-04-25
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