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Clavijo, B.J.* ; Venturini, L.* ; Schudoma, C.* ; Accinelli, G.G.* ; Kaithakottil, G.* ; Wright, J.* ; Borrill, P.* ; Kettleborough, G.* ; Heavens, D.* ; Chapman, H.* ; Lipscombe, J.* ; Barker, T.* ; Lu, F.H.* ; McKenzie, N.* ; Raats, D.* ; Ramirez-Gonzalez, R.H.* ; Coince, A.* ; Peel, N.* ; Percival-Alwyn, L.* ; Duncan, O.* ; Trösch, J.* ; Yu, G.* ; Bolser, D.M.* ; Namaati, G.* ; Kerhornou, A.* ; Spannagl, M. ; Gundlach, H. ; Haberer, G. ; Davey, R.P.* ; Fosker, C.* ; Palma, F.D.* ; Phillips, A.* ; Millar, A.H.* ; Kersey, P.J.* ; Uauy, C.* ; Krasileva, K.V.* ; Swarbreck, D.* ; Bevan, M.W.* ; Clark, M.D.*

An improved assembly and annotation of the allohexaploid wheat genome identifies complete families of agronomic genes and provides genomic evidence for chromosomal translocations.

Genome Res. 27, 885-896 (2017)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Advances in genome sequencing and assembly technologies are generating many high-quality genome sequences, but assemblies of large, repeat-rich polyploid genomes, such as that of bread wheat, remain fragmented and incomplete. We have generated a new wheat whole-genome shotgun sequence assembly using a combination of optimized data types and an assembly algorithm designed to deal with large and complex genomes. The new assembly represents >78% of the genome with a scaffold N50 of 88.8 kb that has a high fidelity to the input data. Our new annotation combines strand-specific Illumina RNA-seq and Pacific Biosciences (PacBio) full-length cDNAs to identify 104,091 high-confidence protein-coding genes and 10,156 noncoding RNA genes. We confirmed three known and identified one novel genome rearrangements. Our approach enables the rapid and scalable assembly of wheat genomes, the identification of structural variants, and the definition of complete gene models, all powerful resources for trait analysis and breeding of this key global crop.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
ISSN (print) / ISBN 1088-9051
e-ISSN 1549-5469
Journal Genome Research
Quellenangaben Volume: 27, Issue: 5, Pages: 885-896 Article Number: , Supplement: ,
Publisher Cold Spring Harbor Laboratory Press
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Research Unit Plant Genome and Systems Biology (PGSB)