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Li, X. ; Fekete, A. ; Englmann, M. ; Götz, C. ; Rothballer, M. ; Frommberger, M. ; Buddrus, K. ; Fekete, J.* ; Cai, C.* ; Schröder, P. ; Hartmann, A. ; Chen, G.* ; Schmitt-Kopplin, P.

Development and application of a method for the analysis of N-acylhomoserine lactones by solid-phase extraction and ultra high pressure liquid chromatography.

J. Chromatogr. A 1134, 186-193 (2006)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
A robust method based on solid-phase extraction (SPE) followed by ultra high pressure liquid chromatography (with trade name of Ultra Performance Liquid Chromatography: UPLC; Waters, Milford, MA, USA) is proposed for the determination of five derivatives of N-acylhomoserine lactones (AHLs) that play a biological role as signal molecules of several gram-negative bacteria. Different commercial SPE cartridges were tested for sample extraction, clean-up and preconcentration. Since the sample matrix was a complex growth media, careful optimization of the SPE with respect to washing procedure, elution solvent and sample solvent was necessary. No sample loss was observed when up to 100 mL spiked full media was added onto the cartridge. Applying UPLC for the determination of AHLs, the performance characteristics of the method showed good separation efficiency and high speed. In order to demonstrate the applicability of the method, supernatants with the known AHL producer Burkholderia cepacia LA3 grown in different media were investigated. Additionally, the method was successfully used for the degradation/uptake study of AHLs from a liquid matrix in which barley was grown under controlled condition. © 2006 Elsevier B.V. All rights reserved.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords N-acylhomoserine lactones; UPLC; solid-phase extraction; method development; quorum sensing
ISSN (print) / ISBN 0021-9673
e-ISSN 1873-3778
Journal Journal of Chromatography A
Quellenangaben Volume: 1134, Issue: 1-2, Pages: 186-193 Article Number: , Supplement: ,
Publisher Elsevier
Publishing Place Amsterdam
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Research Unit Microbe-Plant Interactions (AMP)
Institute of Soil Ecology (IBOE)
Institute of Ecological Chemistry (IOEC)