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Open Access Green as soon as Postprint is submitted to ZB.
Analysis of soil microbial communities based on amplicon sequencing of marker genes.
Biol. Fertil. Soils 53, 485–489 (2017)
The use of cultivation independent methods has revolutionized soil biology in the last decades. Most popular approaches are based on directly extracted DNA from soil and subsequent analysis of PCR-amplified marker genes by next-generation sequencing. While these high-throughput methods offer novel possibilities over cultivation-based approaches, several key points need to be considered to minimize potential biases during library preparation and downstream bioinformatic analysis. This opinion paper highlights crucial steps that should be considered for accurate analysis and data interpretation.
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Publication type
Article: Journal article
Document type
Review
Keywords
16s Rrna ; Amplicon Sequencing ; Bioinformatics ; Next-generation Sequencing ; Sequencing Depth; Pcr Primers; Maize Lines; Diversity; Rhizosphere; Generation; Efficiency
ISSN (print) / ISBN
0178-2762
e-ISSN
1432-0789
Journal
Biology and Fertility of Soils
Quellenangaben
Volume: 53,
Issue: 5,
Pages: 485–489
Publisher
Springer
Publishing Place
New York
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Research Unit Comparative Microbiome Analysis (COMI)