PuSH - Publication Server of Helmholtz Zentrum München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Berninger, M.T.* ; Mohajerani, P. ; Wildgruber, M.* ; Bézière, N. ; Kimm, M.A.* ; Ma, X. ; Haller, B.* ; Fleming, M.J.* ; Vogt, S.* ; Anton, M.* ; Imhoff, A.B.* ; Ntziachristos, V. ; Meier, R.* ; Henning, T.D.*

Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography.

Photoacoustics 6, 37-47 (2017)
Publ. Version/Full Text DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The distribution of intramyocardially injected rabbit MSCs, labeled with the near-infrared dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbo-cyanine-iodide (DiR) using hybrid Fluorescence Molecular Tomography-X-ray Computed Tomography (FMT-XCT) and Multispectral Optoacoustic Tomography (MSOT) imaging technologies, was investigated. Viability and induction of apoptosis of DiR labeled MSCs were assessed by XTT- and Caspase-3/-7-testing in vitro. 2 × 106, 2 × 105 and 2 × 104 MSCs labeled with 5 and 10 μg DiR/ml were injected into fresh frozen rabbit hearts. FMT-XCT, MSOT and fluorescence cryosection imaging were performed. Concentrations up to 10 μg DiR/ml did not cause apoptosis in vitro (p > 0.05). FMT and MSOT imaging of labeled MSCs led to a strong signal. The imaging modalities highlighted a difference in cell distribution and concentration correlated to the number of injected cells. Ex-vivo cryosectioning confirmed the molecular fluorescence signal. FMT and MSOT are sensitive imaging techniques offering high-anatomic resolution in terms of detection and distribution of intramyocardially injected stem cells in a rabbit model.
Impact Factor
Scopus SNIP
Scopus
Cited By
Altmetric
0.000
2.019
15
Tags
Annotations
Special Publikation
Hide on homepage

Edit extra information
Edit own tags
Private
Edit own annotation
Private
Hide on publication lists
on hompage
Mark as special
publikation
Publication type Article: Journal article
Document type Scientific Article
Keywords Cell Labeling ; Fluorescence Molecular Imaging ; Intramyocardial Injection ; Mesenchymal Stem Cells ; Multispectral Optoacoustic Tomography ; Rabbit Heart
Language
Publication Year 2017
HGF-reported in Year 2017
ISSN (print) / ISBN 2213-5979
Journal Photoacoustics
Quellenangaben Volume: 6, Issue: , Pages: 37-47 Article Number: , Supplement: ,
Publisher Elsevier
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)
POF-Topic(s) 30205 - Bioengineering and Digital Health
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-505500-001
PubMed ID 28540184
DOI 10.1016/j.pacs.2017.04.002
Scopus ID 85019168660
Erfassungsdatum 2017-06-22
[➜Report correction]