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Emeny, R.T. ; Baumert, J.J. ; Zannas, A.S.* ; Kunze, S. ; Wahl, S. ; Iurato, S.* ; Knauer-Arloth, J. ; Erhardt, A.* ; Balsevich, G.* ; Schmidt, M.V.* ; Weber, P.* ; Kretschmer, A. ; Pfeiffer, L. ; Kruse, J.* ; Strauch, K. ; Roden, M.* ; Herder, C.* ; Koenig, W.* ; Gieger, C. ; Waldenberger, M. ; Peters, A. ; Binder, E.B.* ; Ladwig, K.-H.

Anxiety associated increased CpG methylation in the promoter of Asb1: A translational approach evidenced by epidemiological and clinical studies and a murine model.

Neuropsychopharmacology 43, 342-353 (2018)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Epigenetic regulation in anxiety is suggested, but evidence from large studies is needed. We conducted an epigenome-wide association study (EWAS) on anxiety in a population-based cohort and validated our finding in a clinical cohort as well as a murine model. In the KORA cohort, participants (n =1522, age 32-72 years) were administered the Generalized Anxiety Disorder (GAD-7) instrument, whole blood DNA methylation was measured (Illumina 450K BeadChip), and circulating levels of hs-CRP and IL-18 were assessed in the association between anxiety and methylation. DNA methylation was measured using the same instrument in a study of patients with anxiety disorders recruited at the Max Planck Institute of Psychiatry (MPIP, 131 non-medicated cases and 169 controls). To expand our mechanistic understanding, these findings were reverse translated in a mouse model of acute social defeat stress. In the KORA study, participants were classified according to mild, moderate, or severe levels of anxiety (29.4%/6.0%/1.5%, respectively). Severe anxiety was associated with 48.5% increased methylation at a single CpG site (cg12701571) located in the promoter of the gene encoding Asb1 (β-coefficient = 0.56 standard error (SE) = 0.10, p (Bonferroni) = 0.005), a protein hypothetically involved in regulation of cytokine signaling. An interaction between IL-18 and severe anxiety with methylation of this CpG cite showed a tendency towards significance in the total population (p =0.083) and a significant interaction among women (p =0.014). Methylation of the same CpG was positively associated with Panic and Agoraphobia scale (PAS) scores (β= 0.005, SE=0.002, p= 0.021, n= 131) among cases in the MPIP study. In a murine model of acute social defeat stress, Asb1 gene expression was significantly upregulated in a tissue-specific manner (p= 0.006), which correlated with upregulation of the neuroimmunomodulating cytokine interleukin 1 beta. Our findings suggest epigenetic regulation of the stress-responsive Asb1 gene in anxiety-related phenotypes. Further studies are necessary to elucidate the causal direction of this association and the potential role of Asb1-mediated immune dysregulation in anxiety disorders.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Posttraumatic-stress-disorder; Cytokine Signaling Socs; Dna Methylation; Panic Disorder; Psychiatric-disorders; Negative Regulators; Depressive-like; Ankyrin Repeat; Gene; Inflammation
ISSN (print) / ISBN 0893-133X
e-ISSN 1470-634X
Journal Neuropsychopharmacology
Quellenangaben Volume: 43, Issue: 2, Pages: 342-353 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Epidemiology II (EPI2)
Institute of Genetic Epidemiology (IGE)
Institute of Computational Biology (ICB)