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Nagarajan, S.* ; Bedi, U.* ; Budida, A.* ; Hamdan, F.H.* ; Mishra, V.K.* ; Najafova, Z.* ; Xie, W.* ; Alawi, M.* ; Indenbirken, D.* ; Knapp, S.* ; Chiang, C.M.* ; Grundhoff, A.* ; Kari, V.* ; Scheel, C. ; Wegwitz, F.* ; Johnsen, S.A.*

BRD4 promotes p63 and GRHL3 expression downstream of FOXO in mammary epithelial cells.

Nucleic Acids Res. 45, 3130-3145 (2016)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Bromodomain-containing protein 4 (BRD4) is amember of the bromo-and extraterminal (BET) domaincontaining family of epigenetic readers which is under intensive investigation as a target for anti-tumor therapy. BRD4 plays a central role in promoting the expression of select subsets of genes including many driven by oncogenic transcription factors and signaling pathways. However, the role of BRD4 and the effects of BET inhibitors in non-transformed cells remain mostly unclear. We demonstrate that BRD4 is required for the maintenance of a basal epithelial phenotype by regulating the expression of epithelialspecific genes including TP63 and Grainy Head-like transcription factor-3 (GRHL3) in non-transformed basal-like mammary epithelial cells. Moreover, BRD4 occupancy correlates with enhancer activity and enhancer RNA (eRNA) transcription. Motif analyses of cell context-specific BRD4-enriched regions predicted the involvement of FOXOtranscription factors. Consistently, activation of FOXO1 function via inhibition of EGFR-AKT signaling promoted the expression of TP63 and GRHL3. Moreover, activation of Src kinase signaling and FOXO1 inhibition decreased the expression of FOXO/BRD4 target genes. Together, our findings support a function for BRD4 in promoting basal mammary cell epithelial differentiation, at least in part, by regulating FOXO factor function on enhancers to activate TP63 and GRHL3 expression.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
ISSN (print) / ISBN 0305-1048
e-ISSN 1362-4962
Journal Nucleic Acids Research
Quellenangaben Volume: 45, Issue: 6, Pages: 3130-3145 Article Number: , Supplement: ,
Publisher Oxford University Press
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Stem Cell Research (ISF)