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de Matos, G.F.* ; Zilli, J.E.* ; de Araújo, J.L.S.* ; Parma, M.M.* ; Melo, I.S.* ; Radl, V. ; Baldani, J.I.* ; Rouws, L.F.M.*

Bradyrhizobium sacchari sp. nov., a legume nodulating bacterium isolated from sugarcane roots.

Arch. Microbiol. 199, 1251–1258 (2017)
DOI PMC
Members of the genus Bradyrhizobium are well-known as nitrogen-fixing microsymbionts of a wide variety of leguminous species, but they have also been found in different environments, notably as endophytes in non-legumes such as sugarcane. This study presents a detailed polyphasic characterization of four Bradyrhizobium strains (type strain BR 10280(T)), previously isolated from roots of sugarcane in Brazil. 16S rRNA sequence analysis, multilocus sequence analysis (MLSA) and analysis of the 16S-23S rRNA internal transcribed spacer showed that these strains form a novel clade close to, but different from B. huanghuaihaiense strain CCBAU 23303(T). Average nucleotide identity (ANI) analyses confirmed that BR 10280(T) represents a novel species. Phylogenetic analysis based on nodC gene sequences also placed the strains close to CCBAU 23303(T), but different from this latter strain, the sugarcane strains did not nodulate soybean, although they effectively nodulated Vigna unguiculata, Cajanus cajan and Macroptilium atropurpureum. Physiological traits are in agreement with the placement of the strains in the genus Bradyrhizobium as a novel species for which the name Bradyrhizobium sacchari sp. nov. is proposed.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Average Nucleotide Identity ; Biological Nitrogen Fixation ; Free-living Nitrogen Fixation ; Multilocus Sequence Analysis ; Non-legumes; Multilocus Sequence-analysis; Natural Endophytic Association; Nitrogen-fixation; Genus Bradyrhizobium; Species Definition; Rhizobium-etli; Nodules; Soils; N-15; Dna
ISSN (print) / ISBN 0003-9276
e-ISSN 1432-072X
Journal Archives of Microbiology
Quellenangaben Volume: 199, Issue: 9, Pages: 1251–1258 Article Number: , Supplement: ,
Publisher Springer
Publishing Place New York
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Research Unit Comparative Microbiome Analysis (COMI)