PuSH - Publication Server of Helmholtz Zentrum München

Kölschbach, J.S. ; Mouttaki, H. ; Pickl, C. ; Heipieper, H.J.* ; Rache, R.* ; Lawson, P.A.* ; Meckenstock, R.U.*

Rectinema cohabitans gen. nov., sp. nov., a rod-shaped spirochaete isolated from an anaerobic naphthalene-degrading enrichment culture.

Int. J. Syst. Evol. Microbiol. 67, 1288-1295 (2017)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
The anaerobic, non-motile strain HMT was isolated from the naphthalene-degrading, sulfate-reducing enrichment culture N47. For 20 years, strain HMT has been a stable member of culture N47 although it is neither able to degrade naphthalene nor able to reduce sulfate in pure culture. The highest similarity of the 16S rRNA gene sequence of strain HMT (89%) is with a cultivated member of the family Spirochaetaceae, Treponema caldarium strain H1T (=DSM 7334T), an obligately anaerobic, thermophilic spirochaete isolated from cyanobacterial mat samples collected at a freshwater hot spring in Oregon, USA. In contrast to this strain and the majority of spirochaete species described, strain HMT showed a rod-shaped morphology. Growth occurred at temperatures between 12 and 50 °C (optimum 37 °C) but the isolate was not able to grow at 60 °C. The strain fermented various sugars including D-glucose, D-fructose, lactose and sucrose. Addition of 0.1% (w/v) yeast extract or 0.1% (w/v) tryptone to the culture medium was essential for growth and could not be replaced by either the vitamin solutions tested or by 0.1% (w/v) peptone or 0.1% (w/v) casamino acids. The DNA G+C content of the isolate was 51.5 mol%. The major fatty acids were C14: 0, C18: 1ω13c, C16: 1ω9t, C16: 1ω11c and C16: 1ω9c. Based on the unique morphology and the phylogenetic distance from the closest cultivated relative, a novel genus and species, Rectinema cohabitans gen. nov., sp. nov., is proposed. The type strain is strain HMT (=DSM 100378T=JCM 30982T).
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Contaminated Sites ; Enrichment Culture N47 ; Naphthalene ; Non-spiral Spirochete
ISSN (print) / ISBN 1466-5026
e-ISSN 1466-5034
Journal International Journal of Systematic and Evolutionary Microbiology
Quellenangaben Volume: 67, Issue: 5, Pages: 1288-1295 Article Number: , Supplement: ,
Publisher SGM
Publishing Place Reading
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Groundwater Ecology (IGOE)