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Loebel, M.* ; Eckey, M.* ; Sotzny, F.* ; Hahn, E.G.* ; Bauer, S.* ; Grabowski, P.* ; Zerweck, J.* ; Holenya, P.* ; Hanitsch, L.G.* ; Wittke, K.* ; Borchmann, P.* ; Rüffer, J.U.* ; Hiepe, F.* ; Ruprecht, K.* ; Behrends, U. ; Meindl, C. ; Volk, H.D.* ; Reimer, U.* ; Scheibenbogen, C.*

Serological profiling of the EBV immune response in Chronic Fatigue Syndrome using a peptide microarray.

PLoS ONE 12:e0179124 (2017)
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Background: Epstein-Barr-Virus (EBV) plays an important role as trigger or cofactor for various autoimmune diseases. In a subset of patients with Chronic Fatigue Syndrome (CFS) disease starts with infectious mononucleosis as late primary EBV-infection, whereby altered levels of EBVspecific antibodies can be observed in another subset of patients. Methods: We performed a comprehensive mapping of the IgG response against EBV comparing 50 healthy controls with 92 CFS patients using a microarray platform. Patients with multiple sclerosis (MS), systemic lupus erythematosus (SLE) and cancer-related fatigue served as controls. 3054 overlapping peptides were synthesised as 15-mers from 14 different EBV proteins. Array data was validated by ELISA for selected peptides. Prevalence of EBV serotypes was determined by qPCR from throat washing samples. Results: EBV type 1 infections were found in patients and controls. EBV seroarray profiles between healthy controls and CFS were less divergent than that observed for MS or SLE. We found significantly enhanced IgG responses to several EBNA-6 peptides containing a repeat sequence in CFS patients compared to controls. EBNA-6 peptide IgG responses correlated well with EBNA-6 protein responses. The EBNA-6 repeat region showed sequence homologies to various human proteins. Conclusion: Patients with CFS had a quite similar EBV IgG antibody response pattern as healthy controls. Enhanced IgG reactivity against an EBNA-6 repeat sequence and against EBNA-6 protein is found in CFS patients. Homologous sequences of various human proteins with this EBNA-6 repeat sequence might be potential targets for antigenic mimicry.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Epstein-barr-virus; Systemic-lupus-erythematosus; Cells In-vivo; Epithelial-cells; Antibody-responses; Lymphoproliferative Disorders; Nasopharyngeal Carcinoma; Rheumatoid-arthritis; Multiple-sclerosis; Peripheral-blood
Language english
Publication Year 2017
HGF-reported in Year 2017
ISSN (print) / ISBN 1932-6203
Journal PLoS ONE
Quellenangaben Volume: 12, Issue: 6, Pages: , Article Number: e0179124 Supplement: ,
Publisher Public Library of Science (PLoS)
Publishing Place Lawrence, Kan.
Reviewing status Peer reviewed
Institute(s) Research Unit Gene Vector (AGV)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Immune Response and Infection
PSP Element(s) G-501500-001
DOI 10.1371/journal.pone.0179124
WOS ID WOS:000403088400028
Scopus ID 85020702009
Erfassungsdatum 2017-07-26
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