PuSH - Publication Server of Helmholtz Zentrum München

Müller, H.M.* ; Schäfer, N.* ; Bauer, H.* ; Geiger, D.* ; Lautner, S.* ; Fromm, J.* ; Riederer, M.* ; Bueno, A.* ; Nussbaumer, T. ; Mayer, K.F.X. ; Alquraishi, S.A.* ; Alfarhan, A.H.* ; Neher, E.* ; Al-Rasheid, K.A.S.* ; Ache, P.* ; Hedrich, R.*

The desert plant Phoenix dactylifera closes stomata via nitrate-regulated SLAC1 anion channel.

New Phytol. 216, 150-162 (2017)
Publ. Version/Full Text Research data DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Date palm Phoenix dactylifera is a desert crop well adapted to survive and produce fruits under extreme drought and heat. How are palms under such harsh environmental conditions able to limit transpirational water loss? Here, we analysed the cuticular waxes, stomata structure and function, and molecular biology of guard cells from P. dactylifera. To understand the stomatal response to the water stress phytohormone of the desert plant, we cloned the major elements necessary for guard cell fast abscisic acid (ABA) signalling and reconstituted this ABA signalosome in Xenopus oocytes. The PhoenixSLAC1-type anion channel is regulated by ABA kinase PdOST1. Energy-dispersive X-ray analysis (EDXA) demonstrated that date palm guard cells release chloride during stomatal closure. However, in Cl(-) medium, PdOST1 did not activate the desert plant anion channel PdSLAC1 per se. Only when nitrate was present at the extracellular face of the anion channel did the OST1-gated PdSLAC1 open, thus enabling chloride release. In the presence of nitrate, ABA enhanced and accelerated stomatal closure. Our findings indicate that, in date palm, the guard cell osmotic motor driving stomatal closure uses nitrate as the signal to open the major anion channel SLAC1. This initiates guard cell depolarization and the release of anions together with potassium.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords S-type Anion Channel ; Slac1 ; Abscisic Acid (aba) Signalling ; Cuticle ; Cuticular Waxes ; Date Palm Phoenix Dactylifera ; Guard Cells ; Stomata; Kinase-phosphatase Pair; Arabidopsis Guard-cells; Abscisic-acid; Plasma-membrane; Subsidiary Cells; Ion-channel; Blue-light; Co2; Protein; Aba
ISSN (print) / ISBN 0028-646X
e-ISSN 1469-8137
Journal New Phytologist
Quellenangaben Volume: 216, Issue: 1, Pages: 150-162 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place Hoboken
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Research Unit Plant Genome and Systems Biology (PGSB)