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Open Access Green as soon as Postprint is submitted to ZB.
Stable-isotope Raman microspectroscopy for the analysis of soil organic matter.
Anal. Bioanal. Chem. 410, 923–931 (2018)
We examined the potential of stable-isotope Raman microspectroscopy (SIRM) for the evaluation of differently enriched C-13-labeled humic acids as model substances for soil organic matter (SOM). The SOM itself can be linked to the soil water holding capacity. Therefore, artificial humic acids (HA) with known isotopic compositions were synthesized and analyzed by means of SIRM. By performing a pregraphitization, a suitable analysis method was developed to cope with the high fluorescence background. Results were verified against isotope ratio mass spectrometry (IRMS). The limit of quantification was 2.1 x 10(-1 13)C/C (tot) for the total region and 3.2 x 10(-2 13)C/C (tot) for a linear correlation up to 0.25 C-13/C (tot). Complementary nanoscale secondary ion mass spectrometry (NanoSIMS) analysis indicated small-scale heterogeneity within the dry sample material, even though-owing to sample topography and occurring matrix effects-obtained values deviated in magnitude from those of IRMS and SIRM. Our study shows that SIRM is well-suited for the analysis of stable isotope-labeled HA. This method requires no specific sample preparation and can provide information with a spatial resolution in the micrometer range.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Raman Microspectroscopy ; Stable Isotopes ; Humic Acids ; Soil Organic Matter; Carbon Nanotubes; Humic Substances; Farmland Soils; Spectroscopy; Microbiology; Temperature; Phosphorus; Reduction; Colloids; Nanosims
ISSN (print) / ISBN
1618-2642
e-ISSN
1618-2650
Quellenangaben
Volume: 410,
Issue: 3,
Pages: 923–931
Publisher
Springer
Publishing Place
Heidelberg
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Groundwater Ecology (IGOE)