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Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
Control of gene editing by manipulation of DNA repair mechanisms.
Mamm. Genome 28, 262-274 (2017)
DNA double-strand breaks (DSBs) are produced intentionally by RNA-guided nucleases to achieve genome editing through DSB repair. These breaks are repaired by one of two main repair pathways, classic non-homologous end joining (c-NHEJ) and homology-directed repair (HDR), the latter being restricted to the S/G2 phases of the cell cycle and notably less frequent. Precise genome editing applications rely on HDR, with the abundant c-NHEJ formed mutations presenting a barrier to achieving high rates of precise sequence modifications. Here, we give an overview of HDR- and c-NHEJ-mediated DSB repair in gene editing and summarize the current efforts to promote HDR over c-NHEJ.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Double-strand Breaks; Homology-directed Repair; Pluripotent Stem-cells; End Resection; Crispr-cas9 Nucleases; Knock-in; Muscular-dystrophy; Mammalian-cells; Mouse Model; Donor Dna
ISSN (print) / ISBN
0938-8990
e-ISSN
1432-1777
Journal
Mammalian Genome
Quellenangaben
Volume: 28,
Issue: 7-8,
Pages: 262-274
Publisher
Springer
Publishing Place
New York
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Developmental Genetics (IDG)