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Xu, Q.* ; Liu, X.* ; Wang, X.* ; Hua, Y.* ; Chen, J.* ; Li, J.* ; Wang, Y.* ; Stöger, T. ; Chen, S.* ; Huang, N.*

Growth arrest-specific protein 7 regulates the murine M1 alveolar macrophage polarization.

Immunol. Res. 65, 1065–1073 (2017)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Growth arrest-specific gene 7 (Gas7) is preferentially expressed in terminally differentiated brain cells and plays a crucial role during neuronal development and neurite outgrowth. Apart from that, Gas7 was found to be abundantly expressed in immune cells like murine macrophage without knowing the actual roles in immune reaction. By using the Illumina microarray analysis, we observed a clear induction of Gas7 but no other Gas family members in murine M1-polarized alveolar macrophage, which was further confirmed by RT-qPCR, Western blotting, and immunostaining analysis, suggesting a likelihood that Gas7 may participate in murine alveolar macrophage polarization. Moreover, we found that the upregulation of Gas7 in M1-polarized alveolar macrophage was almost fully blocked by IKK selective inhibitor BMS, which links Gas7 induction to nuclear factor kappa beta (NF-κB) signaling activation. Interestingly, we found that Gas7 knockdown by small interfering RNA transfection did not affect the pro-inflammatory cytokine gene Tnf and Ilb expression, whereas the expressions of canonic M1 marker gene Nos2 and other M1-dependent genes Il12b, Il6, Cxcl1, Cxcl2, and Cxcl9 were found to be reduced. Furthermore, Gas7-related M1 gene expression in alveolar macrophage was not dependent on NF-κB and STAT1 pathway. Our results demonstrate that Gas7 is potentially involved in regulation of murine M1 alveolar macrophage polarization.  
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Alveolar Macrophage ; Gas7 ; M1 And M2 ; Macrophage Polarization; Gene; Cell; Expression; Receptor; Inflammation; Recruitment; Plasticity; Responses; Pathways; Neurons
ISSN (print) / ISBN 0257-277X
e-ISSN 1559-0755
Journal Immunologic research
Quellenangaben Volume: 65, Issue: 5, Pages: 1065–1073 Article Number: , Supplement: ,
Publisher Springer
Publishing Place New York, NY
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Lung Health and Immunity (LHI)