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Effect of processing intensity on immunologically active bovine milk serum proteins.
Nutrients 9:963 (2017)
Consumption of raw cow's milk instead of industrially processed milk has been reported to protect children from developing asthma, allergies, and respiratory infections. Several heat-sensitive milk serum proteins have been implied in this effect though unbiased assessment of milk proteins in general is missing. The aim of this study was to compare the native milk serum proteome between raw cow's milk and various industrially applied processing methods, i.e., homogenization, fat separation, pasteurization, ultra-heat treatment (UHT), treatment for extended shelf-life (ESL), and conventional boiling. Each processing method was applied to the same three pools of raw milk. Levels of detectable proteins were quantified by liquid chromatography/tandem mass spectrometry following filter aided sample preparation. In total, 364 milk serum proteins were identified. The 140 proteins detectable in 66% of all samples were entered in a hierarchical cluster analysis. The resulting proteomics pattern separated mainly as high (boiling, UHT, ESL) versus no/low heat treatment (raw, skimmed, pasteurized). Comparing these two groups revealed 23 individual proteins significantly reduced by heating, e.g., lactoferrin (log2-fold change = -0.37, p = 0.004), lactoperoxidase (log2-fold change = -0.33, p = 0.001), and lactadherin (log2-fold change = -0.22, p = 0.020). The abundance of these heat sensitive proteins found in higher quantity in native cow's milk compared to heat treated milk, renders them potential candidates for protection from asthma, allergies, and respiratory infections.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Proteomics ; Heat Stability ; Milk Serum Proteins ; Immune-active Proteins; Fat Globule-membrane; Polymeric Immunoglobulin Receptor; Unprocessed Cows Milk; Whey Proteins; Sample Preparation; Childhood Asthma; Heat-treatment; Whole Milk; Skim Milk; Farm
ISSN (print) / ISBN
2072-6643
e-ISSN
2072-6643
Journal
Nutrients
Quellenangaben
Volume: 9,
Issue: 9,
Article Number: 963
Publisher
MDPI
Publishing Place
Basel
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Asthma and Allergy Prevention (IAP)