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Open Access Green as soon as Postprint is submitted to ZB.
A novel Cre-inducible knock-in ARL13B-tRFP fusion cilium reporter.
Genesis 55:e23073 (2017)
Cilia play a major role in the regulation of numerous signaling pathways and are essential for embryonic development. Mutations in genes affecting ciliary function can cause a variety of diseases in humans summarized as ciliopathies. To facilitate the detection and visualization of cilia in a temporal and spatial manner in mouse tissues, we generated a Cre-inducible cilium-specific reporter mouse line expressing an ARL13B-tRFP fusion protein driven by a CMV enhancer/chicken β actin promotor (pCAG) from the Hprt locus. We detected bright and specific ciliary signals by immunostainings of various mono- and multiciliated tissues and by time-lapse live-cell analysis of cultured embryos and organ explant cultures. Additionally, we monitored cilium assembly and disassembly in embryonic fibroblast cells using live-cell imaging. Thus, the ARL13B-tRFP reporter mouse strain is a valuable tool for the investigation of ciliary structure and function in a tissue-specific manner to understand processes, such as ciliary protein trafficking or cilium-dependent signaling in vitro and in vivo.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Arl13b ; Cilia Inducible Transgene ; Ciliopathy ; Cilium Reporter ; Live Cell Imaging Of Cilia; Expression; Disease; Cells; Line
ISSN (print) / ISBN
1526-954X
e-ISSN
1526-968X
Quellenangaben
Volume: 55,
Issue: 11,
Article Number: e23073
Publisher
Wiley
Publishing Place
Hoboken
Non-patent literature
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Reviewing status
Peer reviewed