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Immunohistochemical and flow cytometric analysis of intracellular and membrane-bound Hsp70, as a putative biomarker of glioblastoma multiforme, using the cmHsp70.1 monoclonal antibody.
Methods Mol. Biol. 1709, 307-320 (2018)
The major stress-inducible 70 kDa heat shock (stress) protein 70 (Hsp70) is frequently overexpressed in highly aggressive tumor cells and thus might serve as a tumor-specific biomarker of aggressive disease. We have previously shown that, in contrast to normal cells, tumor cells present Hsp70 on their plasma membrane. In order to elucidate the role of intracellular and membrane-bound Hsp70 as a potential tumor biomarker in glioblastoma multiforme, herein, we describe protocols for the staining of cytosolic Hsp70 in tumor formalin fixed paraffin-embedded (FFPE) sections using immunohistochemistry, and for plasma membrane-bound Hsp70 by multi-parametric flow cytometry using the cmHsp70.1 monoclonal antibody (mAb).
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Flow Cytometry ; Formalin Fixed Paraffin-embedded (ffpe) Sections ; Glioblastoma Multiforme ; Hsp70 Antibody Epitope ; Immunohistochemistry ; Intracellular And Membrane-bound Heat Shock Protein 70 (hsp70)
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Book Volume Title
Chaperones
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 1709,
Pages: 307-320
Publisher
Springer
Publishing Place
Berlin [u.a.]
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Radiation Medicine (IRM)