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Open Access Green as soon as Postprint is submitted to ZB.
Quantitative region-specific DNA methylation analysis by the EpiTYPER™ technology.
Methods Mol. Biol. 1708, 515-535 (2018)
DNA methylation plays a profound role in development and health as well as development and progression of disease. High-throughput quantitative DNA methylation analysis is therefore crucial for the study of the normal physiology of the epigenome and its dysregulation in disease. Many target areas are identified by a range of emerging genome-wide cytosine methylation techniques, but these whole genome scans usually only provide methylation data for a few individual CpG sites (CpGs) within a region. The EpiTYPER™ assay is a region-specific method for the detection and quantitative analysis of DNA methylation that allows performing a high-resolution scan of selected regions. It thus enables a more detailed analysis of single CpGs and the surrounding area and can, in addition to candidate gene methylation analysis, be used to validate CpGs detected by genome wide techniques. The EpiTYPER™ assay allows a fast and reproducible targeted quantification of individual CpGs in a high throughput manner and is based on base-specific cleavage of bisulfite-converted genomic DNA and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Up to 85% of the CpGs within a target region can be analyzed and the detection precision allows quantifying methylation differences as low as 5-7%.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Base-specific Cleavage ; Bisulfite ; Dna Methylation Pattern ; Epityper ; Maldi-tof Ms ; Quantitative
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 1708,
Pages: 515-535
Publisher
Springer
Publishing Place
Berlin [u.a.]
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Epidemiology II (EPI2)