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Schuhmann, K.* ; Srzentić, K.* ; Nagornov, K.O.* ; Thomas, H.* ; Gutmann, T. ; Coskun, Ü. ; Tsybin, Y.O.* ; Shevchenko, A.*

Monitoring membrane lipidome turnover by metabolic15N labeling and shotgun ultra-high-resolution orbitrap fourier transform mass spectrometry.

Anal. Chem. 89, 12857-12865 (2017)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Lipidomes undergo permanent extensive remodeling, but how the turnover rate differs between lipid classes and molecular species is poorly understood. We employed metabolic 15 N labeling and shotgun ultra-high-resolution mass spectrometry (sUHR) to quantify the absolute (molar) abundance and determine the turnover rate of glycerophospholipids and sphingolipids by direct analysis of total lipid extracts. sUHR performed on a commercial Orbitrap Elite instrument at the mass resolution of 1.35 × 10 6 (m/z 200) baseline resolved peaks of 13 C isotopes of unlabeled and monoisotopic peaks of 15 N labeled lipids (Δm = 0.0063 Da). Therefore, the rate of metabolic 15 N labeling of individual lipid species could be determined without compromising the scope, accuracy, and dynamic range of full-lipidome quantitative shotgun profiling. As a proof of concept, we employed sUHR to determine the lipidome composition and fluxes of 62 nitrogen-containing membrane lipids in human hepatoma HepG2 cells.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Crude-oil; Identification; Dynamics; Spectra; Acids
ISSN (print) / ISBN 0003-2700
e-ISSN 1520-6882
Journal Analytical Chemistry
Quellenangaben Volume: 89, Issue: 23, Pages: 12857-12865 Article Number: , Supplement: ,
Publisher American Chemical Society (ACS)
Publishing Place Washington
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Pancreatic Islet Research (IPI)