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Karakatsani, A.* ; Marichal, N.* ; Urban, S.* ; Kalamakis, G.* ; Ghanem, A.* ; Schick, A.J.* ; Zhang, Y.* ; Conzelmann, K.* ; Rüegg, M.A.* ; Berninger, B.* ; de Almodovar, C.R.* ; Gascón, S. ; Kroeger, S.*

Neuronal LRP4 regulates synapse formation in the developing CNS.

Development 144, 4604-4615 (2017)
Publ. Version/Full Text Research data DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
The low-density lipoprotein receptor-related protein 4 (LRP4) is essential in muscle fibers for the establishment of the neuromuscular junction. Here, we show that LRP4 is also expressed by embryonic cortical and hippocampal neurons, and that downregulation of LRP4 in these neurons causes a reduction in density of synapses and number of primary dendrites. Accordingly, overexpression of LRP4 in cultured neurons had the opposite effect inducing more but shorter primary dendrites with an increased number of spines. Transsynaptic tracing mediated by rabies virus revealed a reduced number of neurons presynaptic to the cortical neurons in which LRP4 was knocked down. Moreover, neuron-specific knockdown of LRP4 by in utero electroporation of LRP4 miRNA in vivo also resulted in neurons with fewer primary dendrites and a lower density of spines in the developing cortex and hippocampus. Collectively, our results demonstrate an essential and novel role of neuronal LRP4 in dendritic development and synaptogenesis in the CNS.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Lrp4 ; Central Nervous System Development ; Synapse Formation ; Dendritogenesis ; Transsynaptic Tracing ; Agrin ; In Utero Electroporation ; Psd95 ; Bassoon ; Mouse; Neuromuscular-junction Formation; Receptor-related Protein-4; Hippocampal-neurons; Kinase-ii; Agrin; Expression; Muscle; Cortex; Mechanisms; Plasticity
ISSN (print) / ISBN 0950-1991
e-ISSN 1477-9129
Journal Development / Company of Biologists
Quellenangaben Volume: 144, Issue: 24, Pages: 4604-4615 Article Number: , Supplement: ,
Publisher Company of Biologists
Publishing Place Cambridge
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Stem Cell Research (ISF)