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Detection of the formyl radical by EPR spin-trapping and mass spectrometry.
Free Radical Biol. Med. 116, 129-133 (2018)
For the first time we here present the unambiguous identification of the formyl radical ((CHO)-C-center dot) by EPR (Electron Paramagnetic Resonance) spectroscopy and mass spectrometry (MS) using DMPO (5,5-dimethyl-1-pyrroline N-oxide) as spin trap at ambient temperature without using any catalyst(s). The (CHO)-C-center dot was continuously generated by UV photolysis in closed anoxic environment from pure formaldehyde (HCHO) in aqueous solution. The isotropic hyperfine structure constants of (CHO)-C-center dot were determined as a(N)= 15.72 G and a(H) = 21.27 G. The signals were deconvoluted and split by simulation in their single adduct components: DMPO-CHO, DMPO-H and DMPO-OH. We verified our results at first using MNP (2-methyl-2-nitroso-propane) as spin trap with known literature data and then mass spectrometry. Similarly the MNP adduct components MNP-CHO, MNP-H as well as its own adduct, the MNP-2-methyl-2-propyl (MNP-MP) were deconvoluted. Due to the low signal intensities, we had to accumulate single measurements for both spin traps. Using MS we got the exact mass of the reduced (CHO)-C-center dot adduct independently confirming the result of EPR detection of formyl radical.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Formyl Radical ; Epr ; Simulation ; Mass Spectrometry ; Dmpo ; Ambient Temperature; In-vivo; Hydroxyl Radicals; Resonance; Formaldehyde; Generation; Cells; 5,5-dimethyl-1-pyrroline-1-oxide; Simulation; Photolysis; Mechanism
ISSN (print) / ISBN
0891-5849
e-ISSN
1873-4596
Quellenangaben
Volume: 116,
Pages: 129-133
Publisher
Elsevier
Publishing Place
New York, NY
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Groundwater Ecology (IGOE)