PuSH - Publication Server of Helmholtz Zentrum München

Pekkanen, J.* ; Valkonen, M.* ; Täubel, M.* ; Tischer, C.* ; Leppanen, H.* ; Karkkainen, P.M.* ; Rintala, H.* ; Zock, J.P.* ; Casas, L.* ; Probst-Hensch, N.* ; Forsberg, B.* ; Holm, M.* ; Janson, C.* ; Pin, I.* ; Gislason, T.* ; Jarvis, D.* ; Heinrich, J. ; Hyvärinen, A.*

Indoor bacteria and asthma in adults: A multicentre case-control study within ECRHS II.

Eur. Respir. J. 51:1701241 (2018)
Publ. Version/Full Text DOI PMC
Free by publisher
Open Access Green as soon as Postprint is submitted to ZB.
Both protective and adverse effects of indoor microbial exposure on asthma have been reported, but mostly in children. To date, no study in adults has used non-targeted methods for detection of indoor bacteria followed by quantitative confirmation. A cross-sectional study of 198 asthmatic and 199 controls was conducted within the European Community Respiratory Health Survey (ECRHS) II. DNA was extracted from mattress dust for bacterial analysis using denaturing gradient gel electrophoresis (DGGE). Selected bands were sequenced and associations with asthma confirmed with four quantitative PCR (qPCR) assays. 15 out of 37 bands detected with DGGE, which had at least a suggestive association (p<0.25) with asthma, were sequenced. Of the four targeted qPCRs, Clostridium cluster XI confirmed the protective association with asthma. The association was dose dependent (aOR 0.43 (95% CI 0.22-0.84) for the fourth versus first quartile, p for trend 0.009) and independent of other microbial markers. Few significant associations were observed for the three other qPCRs used. In this large international study, the level of Clostridium cluster XI was independently associated with a lower risk of prevalent asthma. Results suggest the importance of environmental bacteria also in adult asthma, but need to be confirmed in future studies.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Respiratory-health-survey; Real-time Pcr; In-house Dust; Childhood Asthma; Ribosomal-rna; Microbial Exposure; Birth Cohort; Early-life; Children; Population
ISSN (print) / ISBN 0903-1936
e-ISSN 1399-3003
Journal European Respiratory Journal
Quellenangaben Volume: 51, Issue: 2, Pages: , Article Number: 1701241 Supplement: ,
Publisher European Respiratory Society
Publishing Place Sheffield
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Epidemiology (EPI)