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Massner, C. ; Sigmund, F. ; Pettinger, S. ; Seeger, M. ; Hartmann, C.* ; Ivleva, N.P.* ; Niessner, R.* ; Fuchs, H. ; Hrabě de Angelis, M. ; Stelzl, A. ; Koonakampully, N.L. ; Rolbieski, H. ; Wiedwald, U.* ; Spasova, M. ; Wurst, W. ; Ntziachristos, V. ; Winklhofer, M.* ; Westmeyer, G.G.

Genetically controlled lysosomal entrapment of superparamagnetic ferritin for multimodal and multiscale imaging and actuation with low tissue attenuation.

Adv. Func. Mat. 28:1706793 (2018)
Postprint DOI
Open Access Green
Nanomaterials are of enormous value for biomedical applications because of their customizable features. However, the material properties of nanomaterials can be altered substantially by interactions with tissue thus making it important to assess them in the specific biological context to understand and tailor their effects. Here, a genetically controlled system is optimized for cellular uptake of superparamagnetic ferritin and subsequent trafficking to lysosomes. High local concentrations of photoabsorbing magnetoferritin give robust contrast in optoacoustic imaging and allow for selective photoablation of cells overexpressing ferritin receptors. Genetically controlled uptake of the biomagnetic nanoparticles also strongly enhances third-harmonic generation due to the change of refractive index caused by the magnetite-protein interface of ferritins entrapped in lysosomes. Selective uptake of magnetoferritin furthermore enables sensitive detection of receptor-expressing cells by magnetic resonance imaging, as well as efficient magnetic cell sorting and manipulation. Surprisingly, a substantial increase in the blocking temperature of lysosomally entrapped magnetoferritin is observed, which allows for specific ablation of genetically defined cell populations by local magnetic hyperthermia. The subcellular confinement of superparamagnetic ferritins thus enhances their physical properties to empower genetically controlled interrogation of cellular processes with deep tissue penetration.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Magnetic Hyperthermia ; Magnetic Resonance Imaging (mri) ; Magnetoferritin ; Optoacoustic Imaging ; Third-harmonic Generation (thg)
ISSN (print) / ISBN 1616-301X
e-ISSN 1616-3028
Journal Advanced functional materials
Quellenangaben Volume: 28, Issue: 19, Pages: , Article Number: 1706793 Supplement: ,
Publisher Wiley
Publishing Place Weinheim
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)
Institute of Experimental Genetics (IEG)
Institute of Developmental Genetics (IDG)