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Linge, A.* ; Schoetz, U. ; Loeck, S.* ; Lohaus, F.* ; von Neubeck, C.* ; Gudziol, V.* ; Nowak, A.* ; Tinhofer, I.* ; Budach, V.* ; Sak, A.* ; Stuschke, M.* ; Balermpas, P.* ; Roedel, C.* ; Bunea, H.* ; Grosu, A.* ; Abdollahi, A.* ; Debus, J.* ; Ganswindt, U.* ; Lauber, K. ; Pigorsch, S.* ; Combs, S.E.* ; Moennich, D.* ; Zips, D.* ; Baretton, G.B.* ; Buchholz, F.* ; Krause, M.* ; Belka, C. ; Baumann, M.*

Comparison of detection methods for HPV status as a prognostic marker for loco-regional control after radiochemotherapy in patients with HNSCC.

Radiother. Oncol. 127, 27-35 (2018)
Postprint DOI PMC
Open Access Green
OBJECTIVE: To compare six HPV detection methods in pre-treatment FFPE tumour samples from patients with locally advanced head and neck squamous cell carcinoma (HNSCC) who received postoperative (N = 175) or primary (N = 90) radiochemotherapy. MATERIALS AND METHODS: HPV analyses included detection of (i) HPV16 E6/E7 RNA, (ii) HPV16 DNA (PCR-based arrays, A-PCR), (iii) HPV DNA (GP5+/GP6+ qPCR, (GP-PCR)), (iv) p16 (immunohistochemistry, p16 IHC), (v) combining p16 IHC and the A-PCR result and (vi) combining p16 IHC and the GP-PCR result. Differences between HPV positive and negative subgroups were evaluated for the primary endpoint loco-regional control (LRC) using Cox regression. RESULTS: Correlation between the HPV detection methods was high (chi-squared test, p < 0.001). While p16 IHC analysis resulted in several false positive classifications, A-PCR, GP-PCR and the combination of p16 IHC and A-PCR or GP-PCR led to results comparable to RNA analysis. In both cohorts, Cox regression analyses revealed significantly prolonged LRC for patients with HPV positive tumours irrespective of the detection method. CONCLUSIONS: The most stringent classification was obtained by detection of HPV16 RNA, or combining p16 IHC with A-PCR or GP-PCR. This approach revealed the lowest rate of recurrence in patients with tumours classified as HPV positive and therefore appears most suited for patient stratification in HPV-based clinical studies.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Dktk-rog ; Hnscc ; Hpv ; Loco-regional Control ; P16 ; Radiochemotherapy
Language english
Publication Year 2018
HGF-reported in Year 2018
ISSN (print) / ISBN 0167-8140
e-ISSN 1879-0887
Journal Radiotherapy and Oncology
Quellenangaben Volume: 127, Issue: 1, Pages: 27-35 Article Number: , Supplement: ,
Publisher Elsevier
Reviewing status Peer reviewed
Institute(s) Translational Metabolic Oncology (IDC-TMO)
POF-Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Research field(s) Radiation Sciences
PSP Element(s) G-521800-001
DOI 10.1016/j.radonc.2017.12.007
WOS ID WOS:000433102000006
PubMed ID 29295747
Erfassungsdatum 2018-06-20
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