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Hildebrandt, T.B.* ; Hermes, R.* ; Colleoni, S.* ; Diecke, S.* ; Holtze, S.* ; Renfree, M.B.* ; Stejskal, J.* ; Hayashi, K.* ; Drukker, M. ; Loi, P.* ; Göritz, F.* ; Lazzari, G.* ; Galli, C.*

Embryos and embryonic stem cells from the white rhinoceros.

Nat. Commun. 9:2589 (2018)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The northern white rhinoceros (NWR, Ceratotherium simum cottoni) is the most endangered mammal in the world with only two females surviving. Here we adapt existing assisted reproduction techniques (ART) to fertilize Southern White Rhinoceros (SWR) oocytes with NWR spermatozoa. We show that rhinoceros oocytes can be repeatedly recovered from live SWR females by transrectal ovum pick-up, matured, fertilized by intracytoplasmic sperm injection and developed to the blastocyst stage in vitro. Next, we generate hybrid rhinoceros embryos in vitro using gametes of NWR and SWR. We also establish embryonic stem cell lines from the SWR blastocysts. Blastocysts are cryopreserved for later embryo transfer. Our results indicate that ART could be a viable strategy to rescue genes from the iconic, almost extinct, northern white rhinoceros and may also have broader impact if applied with similar success to other endangered large mammalian species.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Intracytoplasmic Sperm Injection; Ceratotherium-simum-simum; Assisted Oocyte Activation; In-vitro; Nuclear Transfer; Important Tool; Semen Quality; Ultrasonography; Cryopreservation; Blastocysts
Language english
Publication Year 2018
HGF-reported in Year 2018
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Journal Nature Communications
Quellenangaben Volume: 9, Issue: 1, Pages: , Article Number: 2589 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Reviewing status Peer reviewed
Institute(s) Institute of Stem Cell Research (ISF)
POF-Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Research field(s) Stem Cell and Neuroscience
PSP Element(s) G-552400-001
DOI 10.1038/s41467-018-04959-2
WOS ID WOS:000437252000002
Scopus ID 85049753966
PubMed ID 29973581
Erfassungsdatum 2018-07-12
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