PuSH - Publication Server of Helmholtz Zentrum München: Analysis of beta-cell function using single-cell resolution calcium imaging in zebrafish islets.

Navigation

Home

Deutsch

Research

Advanced Search

Browse by ...

... Journal

... Publication Type

... Research Data

... Publication Year

Publication overview

Support & Contact

Contact persons

Help

Data protection

Janjuha, S. ; Pal Singh, S.* ; Ninov, N.

Analysis of beta-cell function using single-cell resolution calcium imaging in zebrafish islets.

J. Vis. Exp.:e57851 (2018)

Publ. Version/Full Text

Research data

DOI

PMC

	Open Access Gold (Paid Option)

Abstract
Metrics
Extra information

Pancreatic beta-cells respond to increasing blood glucose concentrations by secreting the hormone insulin. The dysfunction of beta-cells leads to hyperglycemia and severe, life-threatening consequences. Understanding how the beta-cells operate under physiological conditions and what genetic and environmental factors might cause their dysfunction could lead to better treatment options for diabetic patients. The ability to measure calcium levels in beta-cells serves as an important indicator of beta-cell function, as the influx of calcium ions triggers insulin release. Here we describe a protocol for monitoring the glucose-stimulated calcium influx in zebrafish beta-cells by using GCaMP6s, a genetically encoded sensor of calcium. The method allows monitoring the intracellular calcium dynamics with single-cell resolution in ex vivo mounted islets. The glucose-responsiveness of beta-cells within the same islet can be captured simultaneously under different glucose concentrations, which suggests the presence of functional heterogeneity among zebrafish beta-cells. Furthermore, the technique provides high temporal and spatial resolution, which reveals the oscillatory nature of the calcium influx upon glucose stimulation. Our approach opens the doors to use the zebrafish as a model to investigate the contribution of genetic and environmental factors to beta-cell function and dysfunction.

Altmetric

Additional Metrics?

[➜Log in]

Edit extra informations Login

Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Biology ; Issue 137 ; Beta-cells ; Calcium ; Gcamp ; Live-imaging ; Function ; Diabetes ; Zebrafish; Insulin-release; Pancreatic-islets; Glucose; Nitroreductase; Oscillations; Langerhans; Ablation; Ca2+

ISSN (print) / ISBN 1940-087X

e-ISSN 1940-087X

Journal Journal of Visualized Experiments

Quellenangaben Issue: 137, Article Number: e57851

Publisher JoVE

Publishing Place 1 Alewife Center, Ste 200, Cambridge, Ma 02140 Usa

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute for Pancreatic Beta Cell Research (IPI)