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Open Access Green as soon as Postprint is submitted to ZB.
Cloning and characterization of Enterobacter sakazakii pigment genes and in situ spectroscopic analysis of the pigment.
FEMS Microbiol. Lett. 265, 244-248 (2006)
Enterobacter sakazakii is considered an opportunistic foodborne pathogen that is characterized by formation of yellow-pigmented colonies. Because of the lack of basic knowledge about Enterobacter sakazakii genetics, the BAC approach and the heterologous expression of the pigment in Escherichia coli were used to elucidate the molecular structure of the genes responsible for pigment production in Enterobacter sakazakii strain ES5. Sequencing and annotation of a 33.025 bp fragment revealed seven ORFs that could be assigned to the carotenoid biosynthesis pathway. The gene cluster had the organization crtE-idi-XYIBZ, with the crtE-idi-XYIB genes putatively transcribed as an operon and the crtZ gene transcribed in the opposite orientation. The carotenogenic nature of the pigment of Enterobacter sakazakii wt was ascertained by in situ analysis using visible microspectroscopy and resonance Raman microspectroscopy.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Enterobacter sakazakii; bacterial pigment; in situ resonance Raman microspectroscopy
ISSN (print) / ISBN
0378-1097
e-ISSN
1574-6968
Journal
FEMS Microbiology Letters
Quellenangaben
Volume: 265,
Pages: 244-248
Publisher
Wiley
Non-patent literature
Publications
Reviewing status
Peer reviewed