PuSH - Publication Server of Helmholtz Zentrum München: Epstein-barr virus nuclear antigen 2 (EBNA2) gene deletion is consistently linked with EBNA3A,-3B and -3C expression in burkitt's lymphoma cells and with increased resistance to apoptosis.

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Kelly, G.K.* ; Milner, A.E.* ; Tierney, R.J.* ; Croom-Carter, D.S.G.* ; Altmann, M. ; Hammerschmidt, W. ; Bell, A.I.* ; Rickinson, A.B.*

Epstein-barr virus nuclear antigen 2 (EBNA2) gene deletion is consistently linked with EBNA3A,-3B and -3C expression in burkitt's lymphoma cells and with increased resistance to apoptosis.

J. Virol. 79, 10709-10717 (2005)

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Most Epstein-Barr virus (EBV)-positive Burkitt's lymphomas (BLs) carry a wild-type EBV genome and express EBV nuclear antigen 1 (EBNA1) selectively from the BamHI Q promoter (latency I). Recently we identified a distinct subset of BLs carrying both wild-type and EBNA2 gene-deleted (transformation-defective) viral genomes. The cells displayed an atypical "BamHI W promoter (Wp)-restricted" form of latency where Wp (rather than Qp) was active and EBNA1, -3A, -3B, -3C, and -LP were expressed in the absence of EBNA2 or latent membrane proteins 1 and 2. Here we present data strongly supporting the view that the EBNA2-deleted genome is transcriptionally active in these cells and the wild-type genome is silent. Single-cell cloning of three parental Wp-restricted BL lines generated clones carrying either both viral genomes or the EBNA2-deleted genome only, never clones with the wild-type genome only. All rescued clones displayed the Wp-restricted form of latency characteristic of the parent line and retained the original parent cell phenotype. Interestingly, Wp-restricted parent lines and derived clones were markedly more resistant to inducers of apoptosis than standard latency I BL lines. Furthermore, in vitro infection of EBV-negative BL lines with an EBNA2 gene-deleted virus generated EBV-positive converts with Wp-restricted latency and a similarly marked apoptosis resistance. We postulate that, in the subset of BLs displaying Wp-restricted latency, infection of a tumor progenitor cell with an EBNA2 gene-deleted virus has provided that cell with a survival advantage through broadening antigen expression to include the EBNA3 proteins. Copyright © 2005, American Society for Microbiology. All Rights Reserved.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

ISSN (print) / ISBN 0022-538X

e-ISSN 1098-5514

Journal Journal of Virology

Quellenangaben Volume: 79, Issue: 16, Pages: 10709-10717

Publisher American Society for Microbiology (ASM)

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Research Unit Gene Vector (AGV)