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Open Access Green as soon as Postprint is submitted to ZB.
Detection of histone modification dynamics during the cell cycle by MS-based proteomics.
Methods Mol. Biol. 1832, 61-74 (2018)
DNA replication and subsequent deposition of nucleosomes is critical for the maintenance of the genome and epigenetic inheritance. Experiments using human tissue culture cells harvested at defined stages of the cell cycle can help to elucidate the mechanism of histone deposition and chromatin assembly in detail. Here, we describe a pulsed-SILAC approach to distinguish newly synthesized and deposited histones during S-phase of the cell cycle from parental “old” histones incorporated in previous replications and to decipher posttranslational histone modifications (PTMs).
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Histone Quantitation ; Mass Spectrometry ; Ptm-analysis ; Silac Labeling
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 1832,
Pages: 61-74
Publisher
Springer
Publishing Place
Berlin [u.a.]
Reviewing status
Peer reviewed
Institute(s)
CF Metabolomics & Proteomics (CF-MPC)