PuSH - Publication Server of Helmholtz Zentrum München

Kreyling, W.G. ; Möller, W. ; Holzwarth, U.* ; Hirn, S. ; Wenk, A. ; Schleh, C. ; Schäffler, M. ; Haberl, N. ; Gibson, N.* ; Schittny, J.C.*

Age-dependent rat lung deposition patterns of inhaled 20 manometer gold nanoparticles and their quantitative biokinetics in adult rats.

ACS Nano 12, 7771-7790 (2018)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
The increasing use of gold nanoparticles leads to a possible increase of exposure by inhalation. Therefore, we have studied the deposition patterns of inhaled 20 nm gold nanoparticles (AuNP) in 7-90 day old rats and their biokinetics in 60 day old ones. Wistar-Kyoto rats inhaled intratracheally 20 nm Au-195-radiolabeled AuNP by negative pressure ventilation over 2 h. Immediately afterward lungs were excised, inflated and microwave dried. AuNP deposition was analyzed by single-photon emission computed tomography, computed-tomography and autoradiography. Completely balanced, quantitative biodistributions in major organs and all body tissues and total excretion were analyzed from 1 h to 28 d after inhalation. Intratracheal inhalation caused AuNP deposition predominately in the caudal lungs, independent of age. About 30% AuNP were deposited on airway epithelia and rapidly cleared by mucociliary clearance. About 80% of AuNP deposited in alveoli was relocated from the epithelium into the interstitium within 24 h and was inaccessible to broncho-alveolar lavage. During interstitial long-term retention, re-entrainment within macrophages back onto the lung epithelium and to the larynx and gastrointestinal tract (GIT) dominated AuNP clearance (rate 0.03 d(-1)) In contrast, AuNP-translocation across the air-blood barrier was much smaller leading to persistent retention in secondary organs and tissues in the ranking order liver > soft issue > spleen > kidneys > skeleton > blood > uterus > heart > brain. The age independent, inhomogeneous AuNP deposition was probably caused by the negative pressure ventilation. Long-term AuNP clearance was dominated by macrophage-mediated transport from the interstitium to the larynx and GIT. Translocation across the rat air blood barrier appeared to be similar to that of humans for similar sized AuNP.
Altmetric
Additional Metrics?
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Gold Nanoparticle Aerosols ; Intratracheal Inhalation ; Air-blood Barrier Translocation ; Extrapulmonary Nanoparticle Accumulation ; Nanoparticle Clearance ; Alveolar Macrophage ; Interstitial Nanoparticle Relocation ; Epithelial Re-entrainment; Titanium-dioxide Nanoparticles; Pulmonary Macrophagic System; Insoluble Iridium Particles; Long-term Clearance; Ultrafine Particles; In-vivo; Alveolar Macrophages; Acinar Structures; Secondary Organs; Renal Clearance
ISSN (print) / ISBN 1936-0851
e-ISSN 1936-086X
Journal ACS Nano
Quellenangaben Volume: 12, Issue: 8, Pages: 7771-7790 Article Number: , Supplement: ,
Publisher American Chemical Society (ACS)
Publishing Place 1155 16th St, Nw, Washington, Dc 20036 Usa
Non-patent literature Publications
Reviewing status Peer reviewed